ZEISS LSM 800 with Airyscan

Confocal imaging has grown to become the standard choice for most fluorescence microscopy applications. The optical sectioning ability of a confocal imaging system has been brought about by placing a field stop, the pinhole. This application note looks into the details of the recent development and market introduction of the Airyscan detector from ZEISS. Where traditional pinhole and detector design have been reworked to offer greatly improved resolution and signal-to-noise ratio (SNR).

This application note describes how the ZEISS Airyscan can improve resolution and Signal-to-Noise in cryo-fluorescence microscopy, it allows the user to record high-quality cryo- fluorescence data even without immersion optics, thanks to its novel confocal detection scheme.

In this application note ZEISS introduce the features and benefits of the Airyscan detector concept for confocal laser scanning microscopy (LSM). With this feature additional light and spatial information can be collected, improving the spatial resolution and signal to noise ratio.

This application note explains how the Airyscan detector hardware is implemented and describe how the resolution and SNR increase is achieved and how it compares to traditional laser scanning microscopy imaging. Over the last 25 years the technique of confocal imaging has grown to become the standard choice for most fluorescence microscopy applications. The increase in utilization of confocal imaging systems in basic biomedical research can be attributed to the ability of a confocal imaging system to produce optically sectioned images with high contrast while providing acquisition versatility to address many sample and application demands. Over time, novel approaches and options to increase image contrast and instrument versatility have been developed but creating the optical section has not changed.

In this video, Christian Götze, Head of Development at arivis AG, explains a new approach to look at the large data acquired with microscopes. ZEISS and arivis partnered to create a prototype which allows you to render and visualize terabytes of volume data with the Oculus Rift virtual reality headset. You get a deeper insight into very complex brain structures and intuitively understand your data. You can virtually step into your sample, fly through a brain, past brain cells and analyze the image from every angle, and from inside.

Discover how Dr Josh Morgan, Postdoctoral Fellow in the Lichtman Lab at Harvard University, is trying to understand how the nervous system works by looking at how neurons organize their synapses with one another. All behavior in animals depends on cells talking to each other via specific connections, sending out long processes and forming synapses with each other. Understanding the pattern of those connections is one way of understanding animal behavior. Using large scale electron microscopy to reconstruct neural circuits allows you to see every cell and synapse in a circuit and find how they’re wired to one another.

Watch this video to hear Dr Abhinav Singh, Research Associate in Systems Neurophysiology Lab at University of Manchester explain the problem of population coding. When information about the external world is received via sensory signalling, it is transformed by our brain. Exactly how this transformation happens is unknown. Dr Singh is looking into how spikes in the pre-frontal cortex encode information about rule learning, and also discusses the fantastic viewpoint virtual reality offers in data analysis.

Watch this video to discover how Airyscan technology from ZEISS allows better signal-to-noise, resolution and image collection speed. Image areas lost in traditional confocal microscopy with super resolution - even single molecules!

Watch this video to hear Dr. Ralf Engelmann, Product Manager for LSM880 and Airyscan at ZEISS, explain how the LSM880 allows you to extract more information from your samples, allowing higher throughput and in turn, allowed you to image more samples. Extend the sensitivity of your instrument further with Airyscan technology, which allows imaging of areas lost in traditional confocal microscopy with super resolution - you can even image single molecules!

SelectScience presents the Scientists’ Choice Award® for Best New Life Science Product of 2014 to ZEISS Microscopy. The ZEISS LSM 880 with Airyscan was voted as the winning product by scientists around the world, and the award was presented at AACR, in Philadelphia, USA.

This video shows live imaging of a Drosophila brain with triple antibody staining, using the ZEISS LSM 800 with Airyscan. Airyscan gives 1.7 times higher resolution and higher sensitivity than any classic confocal. With the LSM 800, you choose a flexible and compact confocal laser scanning microscope that provides uncompromised image quality.

This video shows cell division of LLC-PK1 (Pig Kidney Epithelial) cells, imaged with the ZEISS LSM 800. Using Airyscan gives 1.7 times higher resolution and higher sensitivity than any classic confocal. With the LSM 800, you choose a flexible and compact confocal laser scanning microscope that provides uncompromised image quality.

A good image often forms the basis for fascinating discoveries. The LSM-800 from Zeiss is a compact confocal which gives you uncompromising image quality. Watch this video to discover how efficient and easy it is to operate the LSM-800.

Enhancing ZEISS Celldiscoverer 7 with ZEISS LSM 900 for optical sectioning

ZEISS and arivis AG partner to provide leading 3D imaging systems

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