TwistAmp® Basic
Gel electrophoresis DNA detection
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Depends on the personality
Pathogenic resources especially in Asia Pacific😱
Need to test the cerebral IQ
Review Date: 20 Oct 2023 | TwistDx
Great product for point-of-care applications
DNA amplification
Great product. Works well and quick compared to PCR. It is ideally suited for point-of-care applications where temperature cycling is not easy.
Review Date: 30 May 2018 | TwistDx
TwistDx is outstanding.
Plants pathogens detection
TwistAmp kit have saved hours in our diagnostic process. Problematic plant DNA extraction and PCR have been reduced to 20 minutes by this kit.
Review Date: 17 Apr 2018 | TwistDx
Excellent, cutting-edge nucleic acid diagnostics reagents.
Basic research
We are very happy to be working with TwistDx's TwistAmp Basic recombinase polymerase amplification kit. The system provides a unique, rapid-format, single-temperature alternative to PCR for amplification of target DNA for use in molecular diagnostics. The company has been very helpful in answering questions about their products and about helping us advance our research on rapid diagnostics of foodborne pathogens.
Review Date: 16 Apr 2018 | TwistDx
Advanced product, alternative to PCR with faster turn-around time !
Diagnostics
User friendly and as effective as PCR but way faster with simple instrumentation.
Review Date: 22 Mar 2018 | TwistDx
Great product we can't do without.
Amplification
Great customer service. TwistDx pellets are a product our scientist can't do without.
Review Date: 12 Mar 2018 | TwistDx
Can't use the results. Maybe the wrong application for these reagents.
Isothermal PCR to detect single nucleotide polymorphisms
The product did not work well for the application that I intended. However, it was unclear whether it was the enzyme cocktail or the primer design that was at fault. I was unable to get allele-specific amplification but rather got amplification of both alleles with each set of primers.
Review Date: 9 Mar 2018 | TwistDx
Manufacturer's Response
Hello Derek, this sounds like a primer design issue. We are always happy to assist our customers troubleshoot, and you can reach out to our experienced technical support team by completing an enquiry form on the contact menu of our website. Best regards, TwistDx
Interesting results. Following up on this technology.
Analyzing DNA content in biological sample
TwistAmp gave me satisfying results. Efficient delivery to France in a few days. Easy to use and detailed protocol included.
Review Date: 8 Dec 2017 | TwistDx
Great product. Quick and sensitive.
Microbiology diagnostics
Product is very easy to use. Straight forward and quick. Exactly what we needed. We are using this kit for on field diagnostic purposes and it works most of the time. This kit can be used in lots of interesting ways eg. sybrgreen incorporation to produce naked-eye visualization. We are satisfied with our purchase and would purchase again.
Review Date: 29 Sept 2017 | TwistDx
OK so far.
Amplification in custom application.
Still figuring out, but early results are promising.
Review Date: 12 May 2017 | TwistDx
The Basic kit contains all enzymes and reagents necessary for the amplification of DNA – all that has to be supplied by the user are the primers and the template.
Features:
- Rapid DNA Amplification
- Single-molecule detection
- Ultra-Sensitive
- 1 x box of 96 reactions
* Twista® device not included
Recombinase Polymerase Amplification: An Isothermal Technique Suitable for a Range of Applications - Evaluation of RPA Volume Tolerance
TwistDx’s proprietary Recombinase Polymerase Amplification (RPA) method addresses many of the challenges faced by PCR methods through a number of benefits, such as the low running temperature. In this application note, RPA performance is demonstrated in reactions established in significantly different total reaction volumes and performed in standard PCR tubes (0.2ml), without oil overlays or any other specialized consumables.
What is Recombinase Polymerase Amplification?
TwistDx’s isothermal nucleic acid amplification technology, recombinase polymerase amplification (RPA), represents a hugely versatile alternative to polymerase chain reaction (PCR) for the development of fast, portable, nucleic acid detection assays. Inherently adaptable to applications as diverse as infectious disease diagnostics and food contamination tests, RPA is ideally suited to field, point-of-care and other settings with minimal resources, and particularly to situations where speed is essential. Easily transportable, user friendly and highly sensitive, RPA is as specific as PCR amplification but is much, much faster. Results are typically generated within 3-10 minutes. And unlike PCR, the RPA reaction doesn’t require thermal or chemical melting, so there’s no need for an expensive thermoycler or any additional equipment or reagents.
Aptamers to Detect Small Traces of Food Allergens
The combination of aptamers and recombinase polymerase amplification (RPA) enables quick, accurate detection of allergens
Novel Nucleic Acid Amplification Method is Ideal for Point-of-Care Applications
Dr. Luis A. Tortajada-Genaro, Polytechnic University of Valencia, discusses recombinase polymerase amplification (RPA) in bioanalytical chemistry
The Mobile Laboratory: How a Scientist Tests for Infectious Diseases out of a Suitcase
Read how Dr. Ahmed Abd El Wahed created a mobile laboratory to keep his research going even in corners of the world with poor infrastructure
Recombinase Polymerase Amplification - The Breakthrough Isothermal Alternative to PCR
TwistDx’s proprietary technology, Recombinase Polymerase Amplification (RPA), is transforming the ability to amplify and detect nucleic acids, in laboratory and resource-limited field settings
