The TruSeq kit is an in-solution sequence capture method for isolating exonic regions of interest in the human genome using hybrid selection. It enables systematic detection of common and rare variants for high-throughput sequencing on any Illumina sequencer at a lower cost per sample.

The TruSeq Exome Enrichment Kit supports cost-effective, scalable exome sequencing studies, offering pre-enrichment sample pooling and the most comprehensive exome coverage, highest uniformity, and lowest DNA input requirement. Master-mixed reagents are coupled with plate-based processing for up to 96 samples and volumes are optimized for liquid handling robots, making the process automation-friendly for even higher throughput.

Prior to exome enrichment, libraries are prepared using the TruSeq DNA Sample Prep Kit, which provides a robust indexing solution for each DNA sample, enabling multi-sample pooling of up to six samples in a single enrichment reaction. Multi-sample pooling dramatically reduces hands-on time compared to other available methods, making large high-throughput studies feasible and economical.