SYNAPT G2-Si HDMS

In this application note, the differentiation of honeys from different botanical origin is demonstrated using an untargeted metabolomics approach via UPLC -MS/MS.

Untargeted metabolomics and lipidomics are hypothesis-generating and exploratory in nature, their purpose is to perform an unbiased screening of metabolites and lipids in biological samples, allowing us to determine patterns or variations between different groups: healthy versus diseased, control versus treated or wild-type versus genetically modified.

This application notebook explains the use of novel and unique chromatographic and spectroscopic tools and methods for metabolomic and lipidomic solutions.

Ion mobility separates ions according to their size and shape, and reports the separation as either the drift time or the collision cross-section (CCS). This application note explores the ion mobility utility of the Waters Vion MS and SYNAPT platforms for CSS analysis of pharmaceuticals.

This poster highlights how mass spectrometric analysis of ex vivo tissue slices from metastatic murine xenograft tumors from LM2-4 cell line with Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) allowed direct comparisons with histology images to determine the molecular profile of necrotic tissues. Showcasing that mass spectrometry is an ideal novel tool for rapid pathology and is highly complementary to current histology based methods widely used in characterization of cancer.

In this collection from Waters, you will find a range of application notes covering the areas of food quality control, research, and safety. Find out how to rapidly screen fatty acids in oil supplements, test for pesticide residues in dried botanicals, and much more.

This is a compilation of application notes from Waters Corporation focused on mass spectrometry imaging and ambient ionization mass spectrometry applied to metabolomics and lipidomics.

This white paper discusses the benefits of gas-phase CCS measurements in HR, accurate-mass UPLC/MS analyses. The rotationally-averaged CCS may be measured experimentally using ion mobility mass spectrometry. CSS is an important distinguishing characteristic of an ion in the gas phase, being related to its chemical structure and three-dimensional conformation. Therefore, it allows the separation of isomers, conformers and promoters and gives increased peak capacity and resolution.

This application note investigates the use of an off-line preparative electrophoresis device to fractionate Host-cell proteins (HCPs) away from the biotherapeutic protein prior to LC-MS analysis. Host-cell proteins (HCPs) are proteins produced by the host cells during recombinant protein drug production. These HCPs contaminate the biotherapeutic drug protein at low levels (1-100 ppm) even after several steps of extensive purification. Since even low level HCPs can stimulate an immunogenic response, regulatory guidelines mandate that these HCPs must be identified and quantified to protect patient safety. Current HCP mass spectrometry (MS) characterization involves some level of pre-fractionation (i.e., electrophoresis, reversed-phase (RP) separation, 2D RP/RP LC, etc.) prior to analysis.

Multi-analyte screening methodologies are essential for monitoring food and environmental samples across the globe. Compliant samples must be eliminated to allow non-compliant samples to be identified for subsequent confirmation and quantitation. This application note describes the ease of use and efficacy of a non-targeted, data independent, analysis type (MSE and HDMSE), coupled with UNIFI.

This application note reports an improvement in protein digestion using a high pressure approach, where promotion of protein denaturation and mechanical stretching of the protein leads to higher digestion efficiency.

Hear how scientists at the National Centre of Excellence in Mass Spectrometry Imaging (NiCE-MSI), National Physical Laboratory, UK, are using a combination of different mass spectrometry techniques to address the Cancer Research UK Grand Challenge to map tumors at a molecular and cellular level. This research will enable the mapping of tumor metabolism in far more detail than ever before and will help to advance both the diagnosis of cancer and the development of new therapies.

Learn how ion mobility mass spectrometry is used to analyze persistent organic pollutants. Watch this video to find out how the technique employs both mass and shape of the complexes being analyzed and how it’s used to discover new pollutants that are harmful.

This video explains a Ohio University project looking at whole-wheat products and how the Waters SYNAPT System is enabling him to define molecules associated with bitterness and, subsequently, provide ideas for how to make nutritious whole-wheat products more palatable.

Michael Batey, Senior Manager Business Development, Waters Corporation, presents a poster by Dr. Zarrine-Afsar, University of Toronto, demonstrating the use of DESI-MSI (Desorption Electrospray Ionization-Mass Spectrometry Imaging) to rapidly detect necrosis in a preclinical model of breast cancer. Speaking at AACR 2017, Michael describes how this technique can identify a biomarker which is specific for necrosis, and its location within the tumor. Michael also presents data which highlights the advantages of DESI-MSI to identify necrosis over traditional histology techniques, such as H&E staining.

Hear how mass spectrometry has enabled researchers at University of Rouen and Total Refining & Chemicals to investigate the physical properties of complex matrices, such as crude oil. Learn how the Waters SYNAPT G2-Si High Definition Mass Spectrometer enables them to perform analyses at the molecular level.

Watch this video to find out more about metabolic profiling and its use in analyzing athletic performance at the Rio Olympic Games in 2016.

Discover how SYNAPT and UPC2 separation technology from Waters is used to study the efficient use of petroleum and environmental effects of oil spills at the Kyungpook National University, South Korea. Find out how this lab analyzes the chemical structure and reactions of petroleum in the environment to help manage oil spills.

Professor Ron M.A. Heeren, Director of the Maastricht MultiModal Molecular Imaging Institute (M4I), Maastricht University, explains how the facility uses advanced molecular imaging technologies to further translational research and make precision medicine a reality. Hear how he is utilizing mass spectrometry to pioneer new techniques for visualizing and understanding the molecular structure of disease tissue. [REIMS Research System with iKnife Sampling is for research use only & is not intended for diagnostic or therapeutic use.]

Watch this video to discover a robust method for the quantitation of whole molecule biopharmaceutical candidates using the Waters iKey separation technology and Synapt G2-Si HDMS system.

Advances in ion mobility mass spectrometry technology is bringing large scale molecular analysis to chemistry, biology, and many areas of medicine. Learn more about research into the development of humans-on-chips, improving drug discovery and screening, and laying the foundations for wider use of precision medicine.

Watch this on-demand webinar to learn how to perform untargeted/semi-targeted metabolomic studies to discover novel biomarkers by mass spectrometry

Expert in mass spectrometry for therapeutic drug monitoring shares research goals and future predictions

Discover the latest techniques to detect and combat doping in sport

Studying proteomics and metabolomics with TOF mass spectrometers from Waters

CGU’s Healthy Aging Research Center focuses on degenerative diseases affecting elderly human health

Hear what the UNIRIO professor has to say