SeQuant® ZIC®-HILIC HPLC Columns

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SeQuant® ZIC®-HILIC HPLC Columns
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ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds. What is HILIC? HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents. In HILIC one has: A column with a hydrophilic stationary phase An eluent with water, buffer and a high concentration of water-miscible organic solvent Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the...Read more

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Average Rating: 4.0

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date: 21 May 2014

  • Application Area: Analytical Chemistry

  • SeQuant® ZIC®-HILIC HPLC Columns

"The column gave good results as expected. The quality of the packed material is really good. The worst thing is the external surface of the container because its made of plastic and it could swell."

Description

ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds.

What is HILIC?

HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents.

In HILIC one has:

  • A column with a hydrophilic stationary phase
  • An eluent with water, buffer and a high concentration of water-miscible organic solvent

Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.

The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions.

  • Improved separation of hydrophilic and polar compounds
  • Orthogonal selectivity to Reversed Phase (RP HPLC)
  • Optimal combination with Mass Spectroscopy (MS)
  • Excellent stability

Product Overview

Product Image

SeQuant® ZIC®-HILIC HPLC Columns

by Merck Millipore

4.0/5.0 (3 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from Merck Millipore for SeQuant® ZIC®-HILIC HPLC Columns