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Member since: 2013
Organization: Idexx Labs
Great Column to have and work with...
Application Area: Analyze metabolites from plasma
"SeQuant ZIC-HILIC columns have wide range of pH. I have used it with ease and the results are highly reproducible. I feel that the price can be lowered. "
Member since: 2013
Organization: Department of Analytical Chemistry, University of Valencia
Application Area:Analytical Chemistry
"The column gave good results as expected. The quality of the packed material is really good. The worst thing is the external surface of the container because its made of plastic and it could swell."
Member since: 2011
Organization: Gedeon Richter Ltd
"Solution for highly polar compounds."
Member since: 2007
ZIC®-HILIC HPLC columns for the separation of all classes of polar and hydrophilic compounds.
What is HILIC?
HILIC or Hydrophilic Interaction Liquid Chromatography is a straightforward chromatographic technique for separation of many types of polar and hydrophilic compounds. To put it simple one can say that HILIC is a normal-phase (NPLC) type of separation but uses reversed-phase (RPLC) type eluents.
In HILIC one has:
Analytes such as amino acids, peptides, carbohydrates, plant extracts and various other polar compounds that usually have little or no retention and may be affected by wettability problems in RPLC, generally have strong retention on the ZIC®-HILIC column.
The zwitterionic ZIC®-HILIC stationary phase is attached to porous silica and is available in columns from capillary to preparative dimensions. The separation is achieved by a hydrophilic partitioning mechanism superimposed on weak electrostatic interactions.