SCIEX OS software

There has been increasing use of high resolution and accurate mass LC-MS/MS systems for drug screening in forensic toxicological settings. This technical note, presents an ultra-fast forensic toxicological screening method using the new SCIEX X500R QTOF system and SCIEX OS 1.0 software. The LC runtime of this method is 2.5 minutes. Two different non-targeted data acquisition methods were used and compared, both of which yielded data that can be analyzed retrospectively.

SCIEX’s X500R QTOF high resolution mass spectrometry system can be used for rapid monitoring of additives in nutritional supplements; after sample injection, a first order mass accuracy number and second order fragmentation spectrum are simultaneously obtained. Currently, over 50 additives can quickly be qualitatively confirmed in this way. Matrix interference in complex matrices can be overcome for specific screening of additives; preprocessing is even simpler and more convenient. The new SCIEX OS software fully integrates instrument control, data collection, data handling, and other processes. The workflow is more intuitive and smarter; this method provides an efficient means for rapid, high-throughput monitoring of nutritional supplements for additives.

Routine characterization of biosimilars or batch comparison of manufactured biologic samples is critical to maintain quality for biotherapeutics throughout development and production. The large size and inherent heterogeneity of biologics make these drugs highly complex, requiring robust mass analysis techniques to ensure accuracy in detection of the protein backbone composition as well as the presence of post-translational modifications. Mass spectrometry is a powerful and flexible tool for biotherapeutic characterization with the ability to analyze both large and small molecules with high mass accuracy. This application note demonstrates the use of a fast, reproducible and robust method for biologic subunit analysis on the X500B QTOF system with streamlined and rapid batch processing and product comparison using the BioPharmaView™ software.

The SCIEX High Resolution Mass Spectrometry X500R QTOF system provides high resolution, high accuracy, high sensitivity and high linearity range scan speeds, making SCIEX SWATH technology not only popular for protein macromolecules but also for small molecule pesticide residue screening. The X500R QTOF system uses newly designed SCIEX OS software to achieve an all-in-one whole process analysis with instrument control, data acquisition and data processing. The software has the built-in SWATH method of setup and powerful automatic deconvolution capabilities. This simple and convenient design meets food safety field use requirements.

Samples of Trastuzumab from two different manufacturing sources were obtained for comparative analysis using peptide mapping. This analysis illustrates what is required for biosimilar characterization, or for analysis of processing and production alterations that may affect the final product. Presented here are the results of the investigation into the differences between the two batches. Also discussed is the ability of the proposed workflows and technologies to rapidly and simply characterize and quantify differences between batches.

POWER UP with SCIEX OS Software, the core engine running SCIEX innovations, now available on the Echo® MS System and the SCIEX Triple Quad™ 7500 LC-MS/MS System – QTRAP® Ready.

Watch a demonstration of the new SCIEX OS software, designed to drive the X500R QTOF System.

In this video, meet the new SCIEX OS, designed to drive the X-Series QTOF mass spec systems. Learn how simple and intuitive the software is for creating new acquisition methods.

New icIEF technology provides a unique and powerful analysis method to address key analytical challenges

Built on usability, efficiency and integrity, SCIEX OS Software promises to power up the latest innovations in mass spectrometry