RNeasy Midi Kit (50)

by QIAGEN | 75144

4.2/5.0 (54 reviews)

Product Image
RNeasy Midi Kit (50)
  • placeholder

RNeasy Midi Kit . For purification of up to 1 mg total RNA from cells, tissues, and yeast.
Kit contents: 50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers. Benefits: Fast procedure delivering high-quality total RNA in minutes. Ready-to-use RNA for high performance in any downstream application. Consistent RNA yields from medium amounts of starting material. No phenol/chloroform extraction. No CsCl gradients, no LiCl or ethanol precipitation
QIAGEN Product Finder
QIAGEN Online shopping guide

Buy this product directly on the QIAGEN website

Reviews

54 Scientists have reviewed this product


Write your own review

Average Rating: 4.2

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date: 10 Dec 2013

  • Application Area: Bullfrog Brain \RNA Isolation

  • RNeasy Midi Kit (50)

"I have used this product for many applications and have found it to be very versatile. The only thing I have had an issue is with the differing amounts of products that they run out at different times and having to overlap kits. "

Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:02 Dec 2013

  • RNeasy Midi Kit (50)

"It has very high purity RNA isolation. The kit is easy to use and directions that come with the kit do not require troubleshooting. The company was very helpful with any questions and overall customer service was very good. Highly recommend for RNA isolation for applications such as qPCR and sequencing technologies. Extremely reliable. "
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:02 Dec 2013

  • Application Area:Immunofluorescence

  • RNeasy Midi Kit (50)

"QIAGEN kit provides a very good yield of DNA and plasmid purification. It also provides a very clean plasmid which doesn't require further purification."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:29 Nov 2013

  • Application Area:RNA Isolation

  • RNeasy Midi Kit (50)

"The kit is very easy to use and directions are clear and helpful! It is very useful in our RNA isolation. It is very good value for the money. "
Rating: 3.7
Ease of use
3 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:29 Nov 2013

  • RNeasy Midi Kit (50)

"It’s easy to use plus has good quality reagents. I believe it is good value for money, quick and versatile to use. It also has good quality read outs. "
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:29 Nov 2013

  • Application Area:Extraction Protocol

  • RNeasy Midi Kit (50)

"It is easy to use, quick and efficient. It has resulted in clean products as confirmed by gel imaging. It has also allowed for extremely high yield and with a reading of absorbance at the 260/280 range of 2.0. "
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:28 Nov 2013

  • Application Area:RNA isolation

  • RNeasy Midi Kit (50)

"It is very self-explanatory, well labelled, and easy to use. It has very ambiguous contents of the substances. It’s very versatile in that each is labelled clearly and the most necessary equipment is included, and also well labelled."
Rating: 3.7
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:07 Oct 2013

  • Application Area:Extraction of viral RNA

  • RNeasy Midi Kit (50)

"A very simple protocol to follow. I have been using this kit for several years now and have had very few failures. I use many of the Qiagen kits and have found them all to be robust and simple to use."
Rating: 2.7
Ease of use
5 out of 5
After sales service
1 out of 5
Value for money
2 out of 5
  • Review date:11 Sep 2013

  • Application Area:Molecular biology

  • RNeasy Midi Kit (50)

"There is no substitute, definitely the best RNA purification kit around. For real, it's all I use, however it could be cheaper."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:06 Aug 2013

  • Application Area:isolation of RNA in cancer cell and tissue

  • RNeasy Midi Kit (50)

"Very good product but so expensive for large scale experiments. Sometimes RNA decay happens. The RNA later reagent does not always work. "
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:06 Aug 2013

  • Application Area:cancer

  • RNeasy Midi Kit (50)

"This kit worked really well for the most part, but I noticed that if I loaded a lot of material onto the column it would clog and I wouldn't get much RNA."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:02 Aug 2013

  • Application Area:cancer research

  • RNeasy Midi Kit (50)

"Straight forward, and easy to use. Works well for cell line experiments and whole organ tissue. Quick isolation process of tissue as well. Very high RNA yield for my tissue of choice (pancreas)."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:Extraction of RNA for gene expression analysis

  • RNeasy Midi Kit (50)

"The kit is very easy to use and the RNA extracted is of high quality. Highly recommend the product."
Rating: 3.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
1 out of 5
  • Review date:03 Jun 2013

  • Application Area:RNA isolation for qRT-PCR

  • RNeasy Midi Kit (50)

"This is a very easy to use kit with minimal (if even) optimization required. Does not come with all reagents, other purchases required. Also has specialized kits for high lipid tissue types. Protocol has been optimized for multiple sample types as well."
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:Tumor Biology

  • RNeasy Midi Kit (50)

"The kit is extremely fast and easy to use.  Yields high quality RNA from cell culture or tissue samples."
Rating: 4.3
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:cDNA synthesis

  • RNeasy Midi Kit (50)

"Isolation of RNA is quite easy and I don't see any DNA contamination because of good columns and buffers. I would like to use this instead of Trizol which is rather risky in terms of purity. I would use other company's product but I am completely satisfied with what I use already."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:cancer research

  • RNeasy Midi Kit (50)

"Quick and easy to use.  Probably the standard for RNA isolation.  The instruction book that comes with the kit is very detailed and answers any question you could have.  The resulting RNA is usually very pure."
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:expression

  • RNeasy Midi Kit (50)

"This kit is easy to use and the results are great. We save time and money because the RNA extracted is always with an excellent RNA Integrity Number and my qPCR Real time always works."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:pcr

  • RNeasy Midi Kit (50)

"Easy to use, very helpful, high yield and high quality as compared to other kits. The best I have even used and the one I am always suggesting. It is the only one I use."
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:03 Jun 2013

  • Application Area:Oncogenetics and Molecular Biology

  • RNeasy Midi Kit (50)

"A high quality of acid nucleic extracted with this kit. An easy and versatile protocol. I also use the Endo-free Maxi prep to extract plasmids to perform a transfection in mammalian cells. I saw a high quality of the material expressed."
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:translational research, small molecules investigation

  • RNeasy Midi Kit (50)

"Since the cost of this and other similar kits from Qiagen has gone a bit higher, we tried to improvise in the lab, trying other possibilities. However, all other protocols and/or kits turned out to be much more difficult to use and with significantly lower yield."
Rating: 3.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
1 out of 5
  • Review date:03 Jun 2013

  • Application Area:Oncology

  • RNeasy Midi Kit (50)

"Kit is very easy to use; simple and fast purification of high-quality RNA from cultured cells.  High RNA recovery.  I have never had the need to contact Qiagen for any after sales services.  The kit isn't as cheap as other kits on the market, but it's a proven product and we're willing to front the extra costs associated with it."
Rating: 3.3
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
2 out of 5
  • Review date:03 Jun 2013

  • Application Area:Fresh Frozen clinical tissue sample prep RNA isolation

  • RNeasy Midi Kit (50)

"The RNeasy midi kit is very easy and straightforward to use. The SOP is very easy to follow and cost efficient for isolating FF tissue sample prep and isolating RNA (for use in Taqman gene expression assays)."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:RNA purification

  • RNeasy Midi Kit (50)

"Pros: It's easier to process compared to Trizol or RNA later. High quality of RNA that is produced. Cons: relatively low yield."
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • RNeasy Midi Kit (50)

"Very good product. Easy to use, reliable, reproducible, very nice. RNA comes out nice, no need to further purify, good for various applications."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:28 May 2013

  • Application Area:plasmid preparation

  • RNeasy Midi Kit (50)

"Very easy to use and get high yield and good quality DNA out. The kit requires less time and is easy to use. You can just follow the protocol to the finish. The system is robust and reliable. You can train a high school student to do it with no problem."
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:16 Apr 2013

  • Application Area:Molecular Biology

  • RNeasy Midi Kit (50)

"This kit has very easy-to-follow instructions and can be used with a tabletop microcentrifuge, no refrigeration required. As such, it's among the easier reactions that I've run. This kit will generate very clean RNA for use in PCR and qPCR reactions (I haven't used it for other things). I used the kit to isolate RNA from tissue and to clean up TRIzol-extracted RNA samples. For cleanup, this kit always worked and generated high quality RNA if I performed all of the optional steps and repeated the final elution twice after letting the elution buffer sit for 1 minute before spinning. If I did not let the elution buffer sit for 1 minute, my yields were lower. I recommend this kit as a great standard kit for RNA extraction and cleanup, with or without TRIzol; however, I feel that Zymo and Omega Bio-tek now offer kits of the same high quality for a lower price. I have not called Qiagen for after-sales services."
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:17 Dec 2012

  • Application Area:Miniprep, cloning

  • RNeasy Midi Kit (50)

"Qiagen has always provided the most reliable kits in my opinion. They are easy and fast and give the highest concentration compared to other plasmid prep kits."
Rating: 3.7
Ease of use
5 out of 5
After sales service
2 out of 5
Value for money
4 out of 5
  • Review date:17 Dec 2012

  • Application Area:mRNA enrichment, mRNA purification after affinity pulldown

  • RNeasy Midi Kit (50)

"The RNeasy RNA isolation and purification kit is fast and yet robust to purify your RNA prior to cDNA synthesis. It is also quite reliable in maintaining a significant yield in your very limited RNA that interacts with your protein (RNA helicase). Furthermore, it requires 10 minutes and you can immediately proceed with your cDNA synthesis."
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:17 Dec 2012

  • Application Area:TRANSCRIPTOME VERIFICATION

  • RNeasy Midi Kit (50)

"I use this kit to extract RNA from plant tissue. This kit is really easy to use, it takes about half an hour from tissue homogenation to final RNA elution fro 8 samples. I have never had any problems with degradation. I have always had good results in finding the transcript isoforms. However I do not really like the customer help from Qiagen because they do not think outside the box with problem solving- it is always that solutions are old. But I would recommend this product for total RNA isolation."
Rating: 3.3
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:17 Dec 2012

  • Application Area:Plant cell development and spermatogenesis

  • RNeasy Midi Kit (50)

"This kit is very easy to use, however the yield for RNA extraction are sometimes lower than expected."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:17 Dec 2012

  • RNeasy Midi Kit (50)

"The instructions are easy to follow even for first time users. It's a great kit for students and people new to DNA purification to use. I love the pre-made buffers because they cut down on work time and variability between samples."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • RNeasy Midi Kit (50)

"The RNeasy mini kit is a really convenient way to quickly purify RNA from all cell types and tissues. I have had a lot of luck obtaining high quality RNA from epithelial cells and keratinocytes which I have used successfully in real time PCR reactions. It is quick, easy and fool-proof."
Rating: 4.3
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
5 out of 5
  • Review date:16 Dec 2012

  • Application Area:RNA purification

  • RNeasy Midi Kit (50)

"This is a very easy to use kit and produces high quality RNA. The produced RNA is suitable for normal RT-PCR or RT-qPCR. It can also be used for mRNA-sequencing and other applications which requires high quality of RNA."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • Application Area:plasmid purification

  • RNeasy Midi Kit (50)

"This kit is easy to use, we can purify plasmid in a very short time with high quality, but the reagent does quickly run out."
Rating: 3.7
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • Application Area:plant cell biology

  • RNeasy Midi Kit (50)

"Gives a very high yield, high quality RNA from plant cells. I would highly recommend this product to anyone who is having problems isolating total RNA from plant cells."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:16 Dec 2012

  • Application Area:Mammalian Cell RNA Isolation

  • RNeasy Midi Kit (50)

"This kit was easy to use. As an undergraduate student who does not have a lot of lab experience, I found this kit to be very straight forward to use. I was able to get great results from this product the very first time I used it. Overall, easy to use and good for beginners."
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:18 Jan 2012

  • Application Area:Cell Biology

  • RNeasy Midi Kit (50)

"Easy to use, manual is clear. Good yield of RNA. Buffers can be bought separately. Consistent results obtained however, in my opinion, it is a bit too expensive."

Description

RNeasy Midi Kit . For purification of up to 1 mg total RNA from cells, tissues, and yeast.
Kit contents: 50 RNeasy Midi Spin Columns, Collection Tubes (15 ml), RNase-free Reagents and Buffers. Benefits: Fast procedure delivering high-quality total RNA in minutes. Ready-to-use RNA for high performance in any downstream application. Consistent RNA yields from medium amounts of starting material. No phenol/chloroform extraction. No CsCl gradients, no LiCl or ethanol precipitation
QIAGEN Product Finder
QIAGEN Online shopping guide

Product Overview

Product Image

RNeasy Midi Kit (50)

by QIAGEN | 75144 | Available Worldwide

4.2/5.0 (54 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Buy this product directly on the QIAGEN website