The rapidSTRIPE E.coli O104 Assay is used for the specific detection of all E.coli O104 strains. Initially, the DNA is isolated from the starting material and used in an amplification reaction which specifically reproduces the gene region for the surface antigen O104. The amplification is combined in the same vessel through a subsequent hybridization reaction with an E.coli O104-specific probe. This reaction format allows the specific detection of those E.coli strains which have the surface antigen O104 and thus prevents the occurrence of false-positive results as a result of a possible mispriming. The detection of the hybridization products then takes place using a lateral flow strip (LFS).
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