QX200™ Droplet Digital™ PCR System (186-4001)

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 3.9/5.0 (2 reviews)

Bio-Rad

Use the QX200 Droplet Digital PCR (ddPCR™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules. Droplet Digital PCR (ddPCR™) is Bio-Rad's uniqu...read more

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Reviews

Afif Abdel Nour


Status:


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Member since 2014

Organization:
King AbdulAziz University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 20 Mar 2014

Application Area: MDx
QX200™ Droplet Digital™ PCR System (186-4001)

"When your qPCR detecting a Cq over 32, the best technique is to use the highly sensitive QX 200 ddPCR."

Read 2 reviews

Valeria Peralta-Leal


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Member since 2013

Organization:
Autonomous University of Tamaulipas
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RATING: 2.7

  • 4 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: Polymorphism
QX200™ Droplet Digital™ PCR System (186-4001)

"It worked well but it needs a better support life. Without it, it doesn't work well enough. I would recommend to other scientists."

Description

 

Use the QX200 Droplet Digital PCR (ddPCR™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules.

Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology. With unrivaled precision, ddPCR provides absolute quantification of target DNA or RNA molecules without the use of standard curves. ddPCR addresses the lack of scalable and practical technologies for digital PCR implementation. The new QX200 ddPCR system puts this powerful technology in your hands, ready to unveil new worlds of research at previously unattainable levels.

Droplet Digital™ PCR system, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications.

The QX200 droplet generator is used to partition ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 droplet reader. Droplets are sipped and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.

Featured at Bio-Rad AACR 2014. Winner of the SelectScience Scientists' Choice Awards Best New Life Sciences Product of 2013.


Features:

  • Flexible Digital PCR Chemistry – optimized for TaqMan hydrolysis probe and EvaGreen applications.
  • Reduced Bias from Amplification Efficiency and PCR Inhibitors.
  • Flexible Assay Setup – scalable for high sensitivity or high throughput.
  • Convenient Assay Design – standard curves are not required.
  • Simple workflow with 96-sample throughput.

Applications:

  • Cancer biomarker studies and copy number variation
  • Pathogen detection
  • Next generation sequencing
  • Gene expression analysis
  • Environmental monitoring
  • Food testing

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