Oasis® µElution Plate
The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, s…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Easy to obtain very clean extract
Purification of urine samples
The Oasis cartridges are very easy to use, robust and reproducible. The selection of an appropriate purification protocol increases the specificity leading to a very clean extract.
Review Date: 4 Jun 2019 | Waters
Great results. Would certainly use this product again in other applications as well.
Purification of metabolites from cell media
The Oasis µplate was used as sample preparation for LC TOF application in order to get a valid concentration determination. The product was very easy to use and produced reliable results and good quality.
Review Date: 9 Oct 2018 | Waters
Good results. Effective.
Solid phase Extraction of biological samples to concentrate the metabolites
The PRIME HLB microElution Plate is a great apparatus for the Solid Phase Extraction technique. I used it to concentrate my metabolites of interest in rat urine samples, and it worked perfectly. But, it didn't work as effectively while concentrating the same metabolites in mice plasma samples. I believe the reproducibility across species (rats vs mice) and the kind of biological samples (plasma vs urine) is low. I would like to see better optimization of the apparatus.
Review Date: 5 Oct 2018 | Waters
Excellent analytical results and ease of use.
Sample preparation for analysis of THC and metabolites in whole blood from impaired drivers
After evaluating many of the SPE products currently on the market on the criteria of recovery, matrix cleanup, ease of use, and cost, Oasis PRiME HLB uElution plate by far is the best option for the analysis of drugs of abuse in whole blood samples. Waters provided excellent support through application notes, in-person training and method development, troubleshooting, and equipment support throughout the optimization and validation process. The uElution plates demonstrate excellent reproducibility, recovery, and matrix cleanup, even with a complex matrix such as whole blood. The 96-well plate form factor will support a lot of scalability for our lab as we receive additional samples, and the Waters positive pressure manifold makes sample processing extremely easy and rapid. Overall, a great system for tricky analytes and matrices!
Review Date: 5 Oct 2018 | Waters
Great results and good limit of detection
Metabolites in urine samples
We are using the Oasis HLB µElution Plate 30µm for our Porphobilinogen analysis in urine and the Oasis MCX µElution plate for our LysoGb3 analysis in urine, on our metabolic lab. We have a manifold voor the extraction system, also from Waters. We use it to concentrate the components to detect them on our Waters Micromass Quattro Micro. We are performing this analysis for a few years now and it gives great results for us. The lysoGb3 analysis is in nmol/l. So the limit of detection is very good for us. We had a private workshop on our lab by some employees from Waters to show us how it all works. So we are satisisfied with it! The plates are not cheap, but they work for us!
Review Date: 15 Aug 2017 | Waters
Review Date: 14 Sept 2009 | Waters
The Oasis® uElution plate format combined with the Oasis chemistries allow elution volumes as low as 25uL, eliminating the time consuming evaporation step, producing very clean extracts with a 5 to 10 fold increase in concentration.
The Oasis® family of sample extraction products is designed to simplify and improve your sample preparation by combining the right sorbent chemistry, device format and methodology. Achieve robust, selective, and sensitive solid-phase extraction [SPE] methods without worrying about low recoveries caused by breakthrough, sorbent drying, pH limitations, and undesirable silanol activity.
Oasis® SPE sorbents—covered by eight U.S. patents—are unique in their purity, reproducibility, stability, and retention characteristics. Today, they are the most widely used polymeric SPE products in bioanalytical laboratories.
A comparison of Extraction Techniques in Bioanalysis and Forensic Toxicology Analyses
In this application note, a comparison was performed between Oasis PRiME HLB SPE, SLE and LLE in both plasma and urine matrices for bioanalysis and forensic toxicology. In plasma, 22 comonly analyzed pharmaceuticals, steroids, and drugs of abuse were extracted using the three aforementioned methods and the results were compared. In urine, 23 drugs of abuse representing opioids, stimulants, benzodiazepines, and synthetic cannabinoid metabolites were tested for forensic toxicology analysis.
The Analysis of Steroid Hormones Using LC-MS/MS
This application note presents an analytically sensitive, accurate and precise measurement of corticosteroids and androgens in serum, for clinical research.
Minimizing Phospholipid Interference During LC-MS/MS Analysis of Vitamin D Metabolites in Serum for Clinical Research
Interferences by matrix components can be challenging when measuring serum levels of vitamin D metabolites by LC-MS/MS, particularly lysophosphatidylcholines. They are challenging to remove during sample preparation and difficult to separate chromatographically. This white paper describes an approach for method optimization using the Oasis PRiME HLB solid phase extraction plate, from Waters.
Waters' Solutions to Peptide and Protein Bioanalysis
The ability to analyze peptides and proteins is becoming increasingly important in the field of biotheraputics. This infographic explains why quantifying large molecules is challenging and how Waters can provide solutions.
LC-MS/MS Analysis of Urinary Benzodiazepines and Z-drugs via a Simplified, Mixed-Mode Sample Preparation Strategy
This application note describes a rapid and simplified solid phase extraction protocol and LC-MS/MS method for the analysis of urinary benzodiazepines and metabolites for forensics toxicology use.
Solid Phase Extraction and Analysis of Coenzyme Q10 from Plasma Using Oasis PRiME HLB for Clinical Research
CoQ10 is a unique hydrophobic molecule that is a key component in ATP synthesis. In this application note, the efficient extraction and analysis of CoQ10 from plasma samples is detailed.
Reducing Sample Volume and Increasing Sensitivity for the Quantification of Human Insulin and 5 Analogs in Human Plasma Using ionKey/MS
This application note demonstrates the need for robust and sensitive analysis of peptide species challenges, both through chromatographic separation and mass spectrometry. Recombinant human insulin and its analogs are perhaps the best known and most widely sold biotherapeutics. Historically, such biologics have been quantified using ligand binding assays. However, specifically in the case of insulin and analogs, these affinity-based assays lack standardization, are subject to matrix effects, and in some cases lack adequate specificity. The use of ionKey/MS facilitated a reduction in sample volume and concomitant increase in sensitivity for the quantification of human insulin and 5 important analogs.
Utilizing Mixed Mode μElution SPE for the LC-MS/MS Analysis of Steroid Hormones in Serum for Clinical Research
This poster evaluates an LC-MS/MS method used to measure serum testosterone and androstenedione enabling investigation of metabolic dysfunction for clinical research purposes. Testosterone, and its precursor, androstenedione, are androgenic steroid hormones that play a central role in the regulation of sexual characteristics.
Simultaneous Analysis of Intact Human Insulin and Five Analogs in Human Plasma Using μElution SPE and a CORTECS UPLC Column
Insulin is perhaps one of the best known and earliest peptide therapeutics. Multiple long and fast-acting analogs have also been developed, and a patient may often be prescribed one of each for diabetes control. Quantification of biologics, such as insulins, has historically been performed using ligand binding assays (LBAs) such as ELISAS. LC/MS/MS, however, has certain advantages over LBAs, such as shorter development times, higher accuracy and precision, the ability to multiplex, no cross-reactivity, and the ability to readily distinguish between closely related insulins. This work provides a single, simple method for the simultaneous, direct quantification of intact human insulin and multiple insulin analogs in human plasma using mixed-mode solid-phase extraction (SPE) and a high-efficiency, solid-core particle column that contains a low-level positive surface charge.
Improving a High Sensitivity Assay for the Quantification of Teriparatide in Human Plasma Using the ionKey/MS System
This study combines μElution SPE and the novel and highly efficient ionKey/MS system to improve a quantitative assay for teriparatide in human plasma. Teriparatide is a recombinant form of a fragment of human parathyroid hormone, used in the treatment of osteoporosis. Peptides in general are often difficult to analyze by LC-MS/MS, as MS sensitivity is low due to the formation of multiple precursors and poor or overly extensive fragmentation. IonKey/MS can provide increased sensitivity compared to 2.1 mm I.D. chromatography with the same injection volume, or equivalent or greater sensitivity with reduced sample consumption, making it ideal for peptide analyses.
Take control over your chromatographic separations: It’s your loss
Discover how to minimize the loss of metal-sensitive analytes and achieve fast analysis and high recovery of oligonucleotides
