Status:
Expert ReviewerMember since: 2012
Organization: UNESP/IBILCE
Application Area: For purification of proteins
"Mono Q 4.6/100 PE (GE Healthcare Life Sciences) is an anion-exchange column used for purification of proteins, peptides, and other biomolecules. The resin is positively charged so the proteins with negative charge bind to it which can then be eluted by a linear or non-linear gradient of salt. The binding capacity is 85 mg/ml resin. I recommend it to use for proteins and peptides purification."
The column is an excellent choice for small-scale polishing in purification of proteins, peptides, and other biomolecules when high purity is required.
The Tricorn columns offer high-resolution separations with greater loading capacity but slightly lower resolution than MiniBeads columns.
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