Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay.
Background Information: The expression of the transcription factors Oct 3/4, Sox2 and Nanog play a critical role in the maintenance of pluripotency and self renewal in stem cells. c-Myc, a member of the helix-loop-helix-leucine zipper family, is a transcription factor that regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. In stem cells it seems to control the balance between stem cell self-renewal and differentiation. Thus the presence of the four proteins Oct-3/4, Sox2, Nanog and c-Myc are indicators for the pluripotency of iPS cells and human embryonic stem cells.
Applications: This MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Panel 1 is a Stem Cell Characterization multiplex assay. The analytes available for this multiplex kit are: c-Myc, Nanog, Oct 3/4, Sox2.