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Description
The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.
The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.
This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.
Customer benefits from the Roche LightCycler® 480 System:
- Speed – rapid temperature control for superb specificity and yield.
The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. - Accuracy – unbiased conditions for unambiguous results
Special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of sample position or differences in reaction volume. - Versatility – utmost flexibility for maximum efficiency
Five excitation and six emission filters of the Roche LightCycler allow you to work with the assay format of your choice, including SYBR Green I, hydrolysis probes, probes, probes, Molecular Beacons, and others. - High-performance optical system from the LightCycler 480
Fluorescence excitation by a high-intensity xenon lamp, and a specially developed signal-collection system, result in maximal sensitivity for any assay format in all channels.
Additional Features of the LightCycler 480 PCR System:
- Customizable modular system concept
Building on the principles of the well-established, performance-proven LightCycler® Software 4.0, the Roche LightCycler® 480 platform comes equipped with modules for run control, Tm calling, and absolute quantification. Further customize your system to your specific needs by adding separately offered advanced analysis modules. - Especially developed reagents
A tailor-made hot-start PCR enzyme master mix is available for each type of LightCycler® 480 application. Built on Roche Diagnostics’ performance proven FastStart technology, these reagents ensure optimal reproducibility and high dynamic ranges. - Perfect integration into laboratory workflow
Compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.
Application notes and news
- LightCycler® 480 and 1536 Real-Time PCR Systems
- Relative Quantification of Multiple mRNA Targets and Reference Genes in Spinocerebellar Ataxia
- Roche Launches New MagNA Pure 96 System: A Perfect Combination of Performance and High Throughput
- Roche Launches Complete Detection Kit for Influenza A /H1N1 on LightCycler® System
- Roche Diagnostics Licenses the Technology used for the LightCycler® System to BIOTECON
- New Technique Involving Roche´s LightCycler® 480 Instrument Enables Efficient and Economic Stud...
- New from Roche: The LightCycler® 480 High Resolution Melting Master for gene scanning
Reviews
Virender Negi Status: See all reviews Member since 2013 Job Title: Research Scientist / Senior / PI Discipline: Biotechnology Work Field: Biotechnology Organization: Beckman Read more » | RATING: 5.0 
"The Light Cycler 480 II is a versatile platform for all the possible Real Time PCR applications with unparalleled speed and accuracy which makes it stand out from the other Real Time PCR systems in the market."   |
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| Andre White Status: See all reviews Member since 2012 Organization: UC Irvine Read more » | RATING: 4.0
"Simple sample loading and analysis. Also, Roche support staff are helpful." |
| JIHUI DU Status: See all reviews Member since 2010 Job Title: Research Scientist / Senior / PI Discipline: Molecular biology Work Field: Hospital Laboratory Organization: SHENZHEN NANSHAN HOSPITAL Read more » | RATING: 4.3
"Easy to use and fast to run a PCR reaction. Gives high accuracy and it has HRM function to use in gene mutation detection." |
| KENNETH TO Status: See all reviews Member since 2010 Organization: THE CHINESE UNIVERSITY OF HONG KONG Read more » | RATING: 4.3
"Easy to use and the software for analysis is convenient." |
| Stan Lightfoot Status: See all reviews Member since 2009 Organization: OUHSC Read more » | RATING: 4.6
"Excellent in our lab, efficient and easy to use." |
| Marc Weinberg Status: See all reviews Member since 2011 Organization: University of North Carolina A Read more » | RATING: 5.0
"Fantastic and easy to use. It can go from start to finish within minutes. Flexible to use with a variety of PCR products. Storage of data allows for safety (no data loss if you choose to analyze in multiple ways). However, with the capacity to hold 96 wells I found the machine to be a little on the large side. The service team offers extensive training before and after you purchase." |
| Antje Bleumke Status: See all reviews Member since 2011 Organization: Hamburg University Read more » | RATING: 4.3
"Works best with Roche Mastermixes. It's easy to handle, only one button to open. However programming is quite difficult, not like a normal PCR cycler." |
| Claudius Stordeur Status: See all reviews Member since 2011 Organization: MLV Halle Read more » | RATING: 3.6
"A fast and reliable system. A very low number of molecules detectable. The system can be used for DNA quantification. The number of copies affect the success of the process." |
| Anne Frentzen Status: See all reviews Member since 2011 Organization: Twincore Read more » | RATING: 4.3
"Equipment and manual are easy to understand and apply, 96 well plate allows analysis of many samples. This system can be used for many applications including SYBR green assays, master hydrolysis probes, genotyping and melling curves. They provide good service in case of any hardware problems." |
| ANA LUISA CORREIA Status: See all reviews Member since 2011 Organization: LAWRENCE BERKELEY NAT'L LAB. Read more » | RATING: 4.6
"Very accurate and user friendly." |
| JESSICA POISSON Status: See all reviews Member since 2011 Organization: UNIV OF NORTH CAROLINA HOSPITALS Read more » | RATING: 4.3
"A fast and very easy-to-use PCR. The closed system is excellent and it is small enough for the bench top." |
| ABDUL SIRAJ Status: See all reviews Member since 2011 Organization: KING FAISAL SPECIALIST HOSPITAL Read more » | RATING: 3.6
"An excellent system for real-time PCR." |
| Randy Scott Status: See all reviews Member since 2011 Organization: BioExpress Read more » | RATING: 5.0
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| Mylan Pho Status: See all reviews Member since 2011 Organization: Genomic Health Read more » | RATING: 4.0
"Very reliable." |
| Holly Green Status: See all reviews Member since 2010 Organization: Dept. Anatomy And Neuroscience Read more » | RATING: 4.3
"Quick and easy to use, good for high through put." |
| Todd Dehm Status: See all reviews Member since 2010 Organization: The Jackson Laboratory Read more » | RATING: 4.0
"The system works really well for melt curve and real-time PCR." |
| Mark Herrmann Status: See all reviews Member since 2010 Organization: ARUP LABORATORIES Read more » | RATING: 5.0
"Versatile instrumentation for genetic analysis." |
| Pedro Lee Status: See all reviews Member since 2010 Organization: Univ California-San Diego Read more » | RATING: 4.3
"A fast, easy-to-use and accurate machine." |
| Jerry Gage Status: See all reviews Member since 2010 Organization: Oclaro Inc Read more » | RATING: 4.0
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| Christine Buerk Status: See all reviews Member since 2008 Organization: Muva Kempten Read more » | RATING: 4.0
"An excellent system." |
| Yin Leng Lee Status: See all reviews Member since 2007 Organization: Mount Sinai School of Medicine Read more » | RATING: 4.3
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| Jaime Agostino Status: See all reviews Member since 2007 Organization: Wadsworth Center Read more » | RATING: 4.0
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| Hong Wu Status: See all reviews Member since 2007 Organization: Wadsworth Center Read more » | RATING: 4.0
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| Hamidou Status: See all reviews Member since 2006 Organization: Tenon Read more » | RATING: 4.6
"Very easy to use." |
| Bouche Naki Status: See all reviews Member since 2006 Organization: ICO Pal Read more » | RATING: 4.0
"Easy to use." |
| Daniel Filho Status: See all reviews Member since 2006 Organization: Shiga University of Medical Science Read more » | RATING: 4.0
"A very good product for Real-time PCR." |
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