LightCycler® 480 Real-Time PCR System

The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.Customer benefits from the Roche LightCycler® 480 System: Speed – rapid temperature control for superb specificity and yield.The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and co...Read more

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Reviews

33 Scientists have reviewed this product


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Average Rating: 4.4

Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date: 30 Jan 2015

  • LightCycler® 480 Real-Time PCR System

"It combines amazing speed with exceptional accuracy which will provides the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis. Another benefit is the good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use. moreover, The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. Finally, we suggest this system because the compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit."

Rating: 4.3
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:27 Oct 2014

  • Application Area:Relative quantification gene expression

  • LightCycler® 480 Real-Time PCR System

"This device is fully sophisticated, GLP based and valuable for work."
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:15 Apr 2014

  • Application Area:Cancer Research

  • LightCycler® 480 Real-Time PCR System

"It is a stable and good instrument for real time PCR, we use it for both diagnostics and research. It’s reliable and easy to learn to users. Both 96 -and 384 wells blocks can be used and are easy to exchange. "
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:10 Feb 2014

  • Application Area:qPCR

  • LightCycler® 480 Real-Time PCR System

"We use the lightcycler 480. It is a great instrument and software. The software allows data analysis while reading."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:10 Feb 2014

  • Application Area:qPCR

  • LightCycler® 480 Real-Time PCR System

"It is much better value for the money than the comparable instrument from Life Tech and better field scientist support. It is very robust and easily integrated with automation. "
Rating: 3.7
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:06 Aug 2013

  • Application Area:diagnostics

  • LightCycler® 480 Real-Time PCR System

"Good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:molecular biology

  • LightCycler® 480 Real-Time PCR System

"This is an easy to use and very reliable instrument. In addition there is excellent support from the company. We found it very helpful in our lab."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • Application Area:molecular biology

  • LightCycler® 480 Real-Time PCR System

"Easy to use, with excellent support. Very reliable and handy.  Easy to develop new assays for."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:13 Jun 2012

  • Application Area:Clinical gene diagnostics

  • LightCycler® 480 Real-Time PCR System

"Easy to use and fast to run a PCR reaction. Gives high accuracy and it has HRM function to use in gene mutation detection."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:05 Dec 2011

  • LightCycler® 480 Real-Time PCR System

"Fantastic and easy to use. It can go from start to finish within minutes. Flexible to use with a variety of PCR products. Storage of data allows for safety (no data loss if you choose to analyze in multiple ways). However, with the capacity to hold 96 wells I found the machine to be a little on the large side. The service team offers extensive training before and after you purchase."
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:03 Nov 2011

  • LightCycler® 480 Real-Time PCR System

"Works best with Roche Mastermixes. It's easy to handle, only one button to open. However programming is quite difficult, not like a normal PCR cycler."
Rating: 3.7
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:28 Oct 2011

  • LightCycler® 480 Real-Time PCR System

"A fast and reliable system. A very low number of molecules detectable. The system can be used for DNA quantification. The number of copies affect the success of the process."
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:20 Oct 2011

  • LightCycler® 480 Real-Time PCR System

"Equipment and manual are easy to understand and apply, 96 well plate allows analysis of many samples. This system can be used for many applications including SYBR green assays, master hydrolysis probes, genotyping and melling curves. They provide good service in case of any hardware problems."

Description

The Roche LightCycler 480 System - Another breakthrough in real-time PCR analysis of gene expression and melting curve-based mutation analysis.

The Roche LightCycler 480 RT-PCR System combines amazing speed with exceptional accuracy and will meet the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis.

This Roche Light Cycler PCR supports all current probe formats and applications and its built-in versatility allows adaptation to future technological advances in genomics research.

Customer benefits from the Roche LightCycler® 480 System:

  • Speed – rapid temperature control for superb specificity and yield.
    The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
  • Accuracy – unbiased conditions for unambiguous results
    Special arrangement of optical components ensures the uniform collection of signals across the plate and makes analysis independent of sample position or differences in reaction volume.
  • Versatility – utmost flexibility for maximum efficiency
    Five excitation and six emission filters of the Roche LightCycler allow you to work with the assay format of your choice, including SYBR Green I, hydrolysis probes, probes, probes, Molecular Beacons, and others.
  • High-performance optical system from the LightCycler 480
    Fluorescence excitation by a high-intensity xenon lamp, and a specially developed signal-collection system, result in maximal sensitivity for any assay format in all channels.

Additional Features of the LightCycler 480 PCR System:

  • Customizable modular system concept
    Building on the principles of the well-established, performance-proven LightCycler® Software 4.0, the Roche LightCycler® 480 platform comes equipped with modules for run control, Tm calling, and absolute quantification. Further customize your system to your specific needs by adding separately offered advanced analysis modules.
  • Especially developed reagents
    A tailor-made hot-start PCR enzyme master mix is available for each type of LightCycler® 480 application. Built on Roche Diagnostics’ performance proven FastStart technology, these reagents ensure optimal reproducibility and high dynamic ranges.
  • Perfect integration into laboratory workflow
    Compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit.

Product Overview

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LightCycler® 480 Real-Time PCR System

by Roche Applied Science - a member of the Roche Group

4.4/5.0 (33 reviews)
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






Receive your quote directly from Roche Applied Science - a member of the Roche Group for LightCycler® 480 Real-Time PCR System

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