Status:
ReviewerMember since: 2021
Organization: University Hospital Center Osijek
I love it!
Application Area:Molecular diagnostics
"Instrument and software are user friendly, easy to use."
Status:
Expert ReviewerMember since: 2013
Organization: GEM Tox Consultants & Labs, Inc.
Reliable, sensitive, modertely-priced qPCR instrument.
Application Area:qPCR
"Very relable, accurate & sensitive instrument for QPCR."
Status:
ReviewerMember since: 2018
Organization: UWC
Fantastic end product.
Application Area:cDNA synthesis, PCR amplification
"I find the instrument easy to set up and use. I find the reagents to be of a very high standard and the end products with very high quality."
Status:
ReviewerMember since: 2015
Organization: UKl Freiburg Neurologie
It is a stable and good instrument for real time PCR
Application Area:THE real time machine - forget the others
"We also use it, alongside a BioRad CFX96 and an ABI 7500. Out of the three, I think the Roche has the best sensitivity, and it also allows you to buy a 384 well block that can be easily interchanged if you want to add high throughput capability. However, the software (or at least the version that we have) is not particularly quick, cannot be loaded on a personal computer, and doesn't have many options for ways to export the data. And when we were interested in expanding into using HRM, we had to cough up a lot of cash to get the upgraded software module which we haven't found to be particularly useful. In contrast, the BioRad CFX is our go-to machine for most 96 well applications. The machine is good, consistent, has never needed maintenance (even though it's often used 6x per day), and has faster ramp times. But the real boon is the software. The basic software is simple to use, can be loaded on everyone's computer for offline analysis, and offers even the difficult normalization options (like normalization to the geometric mean of several reference genes, or ways to normalize across several plates in a multi-plate experiment). It even offers plate wide melt calibration so it gives MUCH more consistent results for anything reliant on melt (like HRM). On balance, the Roche instrument doesn't seem anywhere near superior enough to the BioRad to justify the terrible software. I'd highly recommend taking a look at the BioRad CFX96. It's the one that gets the most use around here!"
Status:
ReviewerMember since: 2015
Organization: Applied Photophyscis
Unmatchable results with LNA probes & an innovation for gene expression studies.
Application Area:Mutation detection & gene quantification
"LightCycler 480 II System is a highly innovative instrument which uses specific optics for having the entire plate analysis simultaneously unlike well-to-well scanning systems producing high errors. LNA probes is a patented technology and offers exceptional specificity. LightCycler 480 II System is industry gold standard in terms of accuracy, reproducibility and repeatability of its results. "
Status:
ReviewerMember since: 2011
Organization: Washington University in St. Louis
Application Area:qPCR
"It is much better value for the money than the comparable instrument from LifeTech and better field scientist support. It is very robust and easily integrated with automation. "
Status:
Expert ReviewerMember since: 2013
Organization: GEM Tox Consultants & Labs, Inc.
Application Area:Analyze Gene and microRNA Expression
"The Roche LightCycler 480 is a PCR instrument that uses either 96 or 384 well plates to carry out automatic measurements on gene expression (or microRNA expression). The instrument can be easily used. It uses melting temperatures to determine the quality and purity of the template. The machine can be serviced by Roche in Indianapolis, IN, and is reasonably priced. I am a fan of Roche and I recommend this product for accurate measurement of gene or micRNA expression."
Status:
ReviewerMember since: 2011
Organization: Faculty of Pharmacy Cairo University
"It combines amazing speed with exceptional accuracy which will provides the needs of a broad range of scientific applications in genomics research including array validation, gene-knockdown studies, and SNP analysis. Another benefit is the good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use. moreover, The LightCycler 480 PCR System utilizes a novel block technology to allow the rapid heating and cooling required to complete a 40-cycle, 384-well quantitative PCR run in less than 40 minutes and leads to extraordinary well-to-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility. Finally, we suggest this system because the compatibility for automated sample-preparation, automated loading and connectivity to laboratory information management systems (LIMS) for integrated workflow monitoring offer uncompromising adaptability for a perfect fit."
Status:
Advanced ReviewerMember since: 2008
Organization: Shahrekord University of Medical Sciences
Application Area:Relative quantification gene expression
"This device is fully sophisticated, GLP based and valuable for work."
Status:
ReviewerMember since: 2009
Organization: Stavanger University Hospital
Application Area:Cancer Research
"It is a stable and good instrument for real time PCR, we use it for both diagnostics and research. It’s reliable and easy to learn to users. Both 96 -and 384 wells blocks can be used and are easy to exchange. "
Status:
ReviewerMember since: 2013
Organization: Washington University in St. Louis
Application Area:qPCR
"We use the lightcycler 480. It is a great instrument and software. The software allows data analysis while reading."
Status:
ReviewerMember since: 2013
Organization: PREDICTIVE BIOSCIENCES
Application Area:diagnostics
"Good reproducibility and accuracy from a quick working instrument. It is very easy to perform optimization experiments and the software is easy to use."
Status:
ReviewerMember since: 2013
Organization: Beilinson Hosp. Tel Aviv Univ.
Application Area:molecular biology
"This is an easy to use and very reliable instrument. In addition there is excellent support from the company. We found it very helpful in our lab."
Status:
ReviewerMember since: 2013
Organization: Beilinson Hosp. Tel Aviv Univ.
Application Area:molecular biology
"Easy to use, with excellent support. Very reliable and handy. Easy to develop new assays for."
Status:
ReviewerMember since: 2010
Organization: SHENZHEN NANSHAN HOSPITAL
Application Area:Clinical gene diagnostics
"Easy to use and fast to run a PCR reaction. Gives high accuracy and it has HRM function to use in gene mutation detection."
Status:
ReviewerMember since: 2011
Organization: University of North Carolina A
"Fantastic and easy to use. It can go from start to finish within minutes. Flexible to use with a variety of PCR products. Storage of data allows for safety (no data loss if you choose to analyze in multiple ways). However, with the capacity to hold 96 wells I found the machine to be a little on the large side. The service team offers extensive training before and after you purchase."
Status:
ReviewerMember since: 2011
Organization: Hamburg University
"Works best with Roche Mastermixes. It's easy to handle, only one button to open. However programming is quite difficult, not like a normal PCR cycler."
Status:
ReviewerMember since: 2011
Organization: MLV Halle
"A fast and reliable system. A very low number of molecules detectable. The system can be used for DNA quantification. The number of copies affect the success of the process."
Status:
ReviewerMember since: 2011
Organization: Twincore
"Equipment and manual are easy to understand and apply, 96 well plate allows analysis of many samples. This system can be used for many applications including SYBR green assays, master hydrolysis probes, genotyping and melling curves. They provide good service in case of any hardware problems."
Status:
ReviewerMember since: 2011
Organization: UNIV OF NORTH CAROLINA HOSPITALS
"A fast and very easy-to-use PCR. The closed system is excellent and it is small enough for the bench top."
Additional Features of the LightCycler 480 PCR System:
Manufacturer Roche Applied Science - a member of the Roche Group
4.4 / 5.0 | 40 reviews