LightCycler ® 2.0 Instrument
Roche Applied Science - a member of the Roche Group
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 col...read more
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Member since 2014
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"I've used the carousel based PCR system extensively for about a year. The set up of reagents are easy and for the most part pretty fast. We used 20ul reactions and our runs on averagely took us about 35 minutes. The biggest drawback to this system was that the capillaries you load your sample in are glass. Occasionally one would break due to miss handling or just weak glass. Setting up a new run wasn't a problem but we would have to bleach everything down to prevent contamination. Other than that I really enjoy running PCR on this product. "
The LightCycler® 2.0 Instrument allows you to monitor amplification of 32 PCR products simultaneously, in real-time and online, with six different detection channels. The LightCycler® Software 4.1 collects fluorescence data from all six channels at each cycle and displays it immediately. This enables you to terminate PCR runs early if signal levels are adequate, or prolong them when signals are too low. Amplification occurs in specially designed reaction capillaries. Their superior surface-to-volume ratio provides extremely rapid thermal transfer, which facilitates high-speed thermal cycling. The combination of LightCycler® Capillaries and the air-driven temperature control of the LightCycler® 2.0 Instrument ensures rapid PCR. An entire 35-cycle run can be performed in as little as 30 minutes (with 20 μl capillaries) to 60 minutes (with 100 μl capillaries).
Fluorescence detection formats: The LightCycler® 2.0 Instrument is optimized for two fluorescence detection formats: SYBR Green I, HybProbe probes, and hydrolysis probes. In addition, the instrument supports a wide variety of other fluorescence detection formats, such as monocolor SimpleProbe probes and other formats based on FRET (fluorescence resonance energy transfer). However, it is essential that the fluorescent dyes used are compatible with the LightCycler® 2.0 optical unit.
Software: The LightCycler® 2.0 Instrument utilizes the LightCycler® Software 4.1, an optimized version of the LightCycler® 4.0 and 4.05 Software, which provides advanced data-analysis modules, efficient data management, and effective data protection.