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Member since: 2015
Organization: University of Manchester
The new gSTED offers great resolution improvement in xy and z planes
Application Area: Super resolution fluorescence imaging
"We have the new (summer 2015) gated STED. Brilliant microscope for imaging fixed samples with greatly enhanced resolution in xy and z. The new gSTED is a huge improvement on the earlier versions which bleach the sample. My lab loves this microscope!"
The Leica TCS SP8 STED super-resolution microscope allows you to discover more and provides fast, intuitive, purely optical access to structural details smaller than 50 nm.
Studying subcellular architecture and dynamics with conventional fluorescence microscopy can be challenging as structures smaller than 200 nm are lost. Stimulated Emission Depletion (STED) microscopy is a stunningly simple way to overcome the diffraction limit - where fluorescence is generated is scaled down to sub-diffraction size. Stimulated emission switches off the ability of the dye to fluoresce in the periphery of the excitation spot yielding confocal super-resolution pixel by pixel.
STED microscopy does not rely on mathematics. What you see is what you get. Of course, results can be improved further by deconvolution. Just one click instantly switches from confocal to super-resolution, even during a live scan. Within seconds, imaging parameters can be optimized and super-resolved images obtained.
Leica TCS SP8 STED Benefits: