STED (STimulated Emission Depletion) microscopy provides fast, intuitive, and purely optical access to study subcellular architecture and dynamics at the nanoscale. STED super-resolution meets the requirements of daily research and enables to discover minute details with live cell imaging capabilities. TCS SP8 STED 3X, allows the whole spectrum of visible light and opens the door to super-resolution in all dimensions.
With TCS SP8 STED 3X, two light paths are available at the instrument that generate different STED patterns. The vortex phase mask creates the classic STED donut which improves resolution only in x and y. A second light path with a different phase mask forms the z donut that increases resolution also in z. STED light can be steplessly distributed between the two paths.
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