KASP Genotyping Chemistry

by LGC

4.2/5.0 (4 reviews)

KASP delivers outstanding assay robustness and accuracy with significant cost savings.

The patented KASP™ genotyping method is a homogeneous, FRET-based end-point PCR assay that enables accurate bi-allelic discrimination of known SNPs and InDels. Unlike other PCR-based genotyping assays, KASP requires no labelling of the target-specific primers or probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large / mega InDels. The KASP genotyping technology was developed for use in our own service laboratories where it has been in use for over 10 years, generating over 1 billion datapoints. Today KASP has nearly 2,000 published references. Whether purchased as a product for use in your laboratory, or as a genotyping service at our state of th...Read more

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Reviews

4 Scientists have reviewed this product


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Average Rating: 4.2

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date: 26 Aug 2014

  • Application Area: Genotyping

  • KASP Genotyping Chemistry

"Super fast method for analyzing large numbers of samples. This product is helpful for analyzing segregating population on a large scale. For example, searching for the EMS mutated candidate gene responsible for the phenotype in segregating population after outcross. Only 3 components (DNA of interest, KASP reagent and KASP assay) required, therefore very easily done in 96 well plate. Easy to analyze the results. Mostly no false positive observed. One of best, quick and easy assay in my PhD work."

Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:22 Aug 2014

  • Application Area:RESEARCH

  • KASP Genotyping Chemistry

"Very easy to work with. Data is available way before due data. The representatives are very knowledgeable and prices are unbeatable. "
Rating: 3.3
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:21 Aug 2014

  • Application Area:Biotechnology

  • KASP Genotyping Chemistry

"Easy-to-use product for high-throughput genotyping. Advantages: Standard protocol for all SNPs, cheaper than TaqMan probes and shorter sequences needed. Disadvantages: False positives should be optimized."

Description

The patented KASP™ genotyping method is a homogeneous, FRET-based end-point PCR assay that enables accurate bi-allelic discrimination of known SNPs and InDels.

Unlike other PCR-based genotyping assays, KASP requires no labelling of the target-specific primers or probes, giving it a clear cost advantage. It also allows greater flexibility for assay design, which translates into a higher overall success rate and the unique ability to genotype large / mega InDels.

The KASP genotyping technology was developed for use in our own service laboratories where it has been in use for over 10 years, generating over 1 billion datapoints. Today KASP has nearly 2,000 published references. Whether purchased as a product for use in your laboratory, or as a genotyping service at our state of the art genotyping laboratories in Europe and North America, KASP genotyping delivers accurate results and significant cost savings so customers can get the most out of their research budget.

KASP genotyping is based on an open format PCR workflow. There is no need to buy new expensive equipment to be able to run KASP in your own laboratory; completed KASP reactions can be read on most FRET-capable plate readers and qPCR instruments.

 

Key features:

  • Accuracy: >99.8% based on independent assessment
  • Industry leading SNP & InDel assay conversion rate (>90%)
  • Flexible primer design which increases the rate of successful assay development
  • Supports low, medium and high-throughput studies and individual repeat assays
  • Compatibility with a wide range of liquid handling systems and thermal cyclers, signal can be read on most FRET-capable plate readers and qPCR instruments.
  • Breakthrough cost savings

 

Applications:
Many applications in plant, animal, human and microbial research

Plant

  • Marker-assisted selection for crop improvement (including MARS and MABC)
  • Fingerprinting / parental screening
  • SNP validation
  • Field trial genotyping

Animal

  • Marker-assisted selection for livestock breeding
  • Speciation
  • Sample provenance and quality control
  • Mapping traits within disease models and breeding populations (e.g. zebrafish, mouse, rat)
  • Speed congenics

Human

  • Genetic variant analysis for human disease research
  • Cohort studies
  • Disease SNP replication/validation studies

Product Overview

Product Image

KASP Genotyping Chemistry

by LGC | Available Worldwide

4.2/5.0 (4 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






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