iTaq™ Universal SYBR® Green One-Step Kit (172-5150)
The iTaq™ universal SYBR® Green one-step kit is a fast and convenient solution for real-time PCR.
The powerful combination of iScript™ RNase H+ MMLV reverse transcriptase and hot-start iTaq DNA polymerase in one reaction, provides improved PCR efficiency, wider dynamic range, superior sensitivity and specificity, and inhibitor tolerance — even with cell lysates — without affecting performance.
iTaq™ Universal SYBR® Green One-Step Kit (172-5150) Features:
- Enhanced efficiency, specificity, and sensitivity — our patented* inhibitor reducer prevents the interference of the RT enzyme, which leads to reduced sensitivity and efficiency, with the DNA polymerase during the replication of the cDNA.
- Use any ROX dependent or independent real-time PCR systems — the universal reference dye in this supermix enables ROX normalization of qPCR data regardless of the ROX level requirements of the qPCR system.
- Achieve superior gene expression results under various cycling conditions — our robust formulation delivers consistent performance in standard or fast cycling conditions, primer concentrations, sample and target types, and temperature ranges, allowing cost and time savings.
- Obtain better results with qPCR assays — simplify your workflow and experimental design using our laboratory-validated PrimePCR™ assays.