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Member since: 2009
Application Area: Analysis of ammonium in Tobacco related Samples
"Our objectives are to improve the efficiency and resolution of the HPLC method for ammonium analysis and reduce the analysis time and cost. A high capacity and high efficiency cation exchange column (IonPac CS16) with smaller particle size and smaller column inner diameter was used in our new method. The ammonium peak can be integrated easily and automatically by the instrument software, due to sharper ammonium peak and better separation from interfering peaks, thus saving the analyst time for integrations. These features of the new method (higher resolution, sharper peak and easy integration) will also contribute to the better accuracy and precision of the method. This method has been applied for all ammonia analysis (mainstream and sidestream tobacco smoke samples, smokeless tobacco and whole tobacco samples). It is good for high throughput sample analysis, as it can analyze diluted samples with reduced instrument maintenance and longer column and suppressor lifetime (since column "