In-Fusion® HD Cloning Plus PCR Cloning Kits by Takara Bio

Manufacturer Takara Bio  |   Model: 6389  |  Available Worldwide
4.9
/
5.0
  |  6 reviews
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing—Single-Cell Transcriptome Analysis with Ultimate Sensitivity


In-Fusion<sup>®</sup> HD Cloning Plus PCR Cloning Kits by Takara Bio product image
In-Fusion® HD Cloning Plus PCR Cloning Kits
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Average Rating: 4.9
6 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


Easy to use
Rating: 5.0

  • Application Area: Mutant deletion cloning

"Makes design and workload much easier. Really like the online tool."

Review date: 12 Dec 2017 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Buenos Aires



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great cloning strategy
Rating: 5.0

  • Application Area:Molecular Biology

"This kit turns out to be very easy to use. It saved the cloning of a difficult and long cDNA that we needed and couldn't clone with regular methods."

Review date: 06 Feb 2017 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Gurdon Institute, Cambridge University.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results with the In-fusion cloning kit
Rating: 5.0

  • Application Area:Plasmid cloning

"It was very simple and effective to use. It’s easy to design and clone. It works like a magic mixture. "

Review date: 12 Jul 2016 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: University of Milan Bicocca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Cloning

"This kit is perfect for cloning larger fragment up to 4kb in first attempt. I am using this kit from last year and it’s perfect for cloning larger construct. The sensitivity is amazing and efficiency of positive clones is above 70%. I highly recommend because of its capability and sensitivity, saved lot of time as compared to normal cloning and decreases repetitive experiment as indirectly save cost as well. When I started using it was new to me as I failed with other cloning product at last I came up with this kit and worked very well since. "

Review date: 13 Oct 2015 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: University Freiburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Gateway-cloning of BCR constructs

"Cloning on the principle of a fire-and-forget system. Fast and easy application in combination with efficiencies of <90% positive clones. Worth to change.... "

Review date: 21 May 2015 | In-Fusion® HD Cloning Plus PCR Cloning Kits
  • Status:

    Reviewer

  • Member since: 2008

  • Organization: UT Health Science Center



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.3
"The best for cloning large sequence inserts."

Review date: 10 Aug 2008 | In-Fusion® HD Cloning Plus PCR Cloning Kits

SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing—Single-Cell Transcriptome Analysis with Ultimate Sensitivity

The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth-generation of SMARTer Ultra Low kits, and our most sensitive mRNA-seq kit for single cells and ultra-low inputs. This kit takes SMART (Switching Mechanism at 5’ End of RNA Template) technology to the next level by incorporating and improving upon the SMART-Seq2 method and locked nucleic acid (LNA) technology. With these additions—referred to as "SMARTer-Seq® chemistry"—this kit is able to outperform both the SMART-seq2 method and previous generations of SMARTer kits by identifying the greatest number of transcripts. The Clontech tradition of continuous innovation extends to this kit by providing direct cDNA synthesis from as few as 1–1,000 intact cells (or as little as 10 pg–10 ng of total RNA), high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts. The full-length cDNA libraries from this kit are compatible with both Illumina® and Ion Torrent sequencing platforms.

The core SMART technology in this kit is an immensely powerful tool for studying the full transcriptome, including splice junctions and alternative splicing events in cells, and provides full-length gene coverage. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells. LNA technology improves the efficiency of template switching, which increases the number of genes identified to higher levels than any other SMARTer Ultra Low kit for mRNA-seq.

Information about prior SMARTer Ultra Low RNA kits can be found here 

Features:

  • SMART-Seq2 and LNA technologies—Template switching efficiency is improved by building on LNA technology
  • Unparalleled sensitivity—Start with as little as 1 cell or 10 pg of total RNA (input range: 1–1,000 cells or 10 pg–10 ng of total RNA)
  • High-quality RNA-seq data—Identify the highest number of genes of any SMARTer Ultra Low kit; maintain full-length gene information, low percentage of rRNA reads, and great representation of GC-rich transcripts
  • Suitable for multiple sequencing platforms—cDNA libraries are compatible with either Illumina or Ion Torrent NGS platforms
  • Sample prep made easy—The single-tube protocol works directly on whole cells and helps to preserve sample integrity
  • Interested in more data and FAQs about this product? Visit the NGS Resource Portal 

Applications:

  • cDNA synthesis from small quantities of cells or total RNA for transcriptome sequencing
  • cDNA output from this kit can be used with either our Low Input Library Prep Kit or Nextera® XT library preparation kits for Illumina sequencing, or with the Ion Xpress Plus Fragment Library Kit for Ion Torrent sequencing

Product Overview

In-Fusion<sup>®</sup> HD Cloning Plus PCR Cloning Kits by Takara Bio product image

In-Fusion® HD Cloning Plus PCR Cloning Kits

Manufacturer Takara Bio  |  Available Worldwide

4.9 / 5.0 | 6 reviews