IMAP® TR-FRET Assays provide a non-radioactive, homogeneous and proprietary antibody-free platform for accurate determination of kinase, phosphatase and phosphodiesterase activities. Each assay utilizes a simple mix and read method to deliver a robust fluorescence signal that is amenable to miniaturization for greater cost savings during high throughput screens. IMAP Assay Kits are designed for researchers who have their own enzyme and substrate, or want to purchase substrates from our extensive validated substrate lists, including PDE, Ser/Thr 2 (CMGC/CK1/STE/TKL), Ser/Thr 1 (CAMK/AGC) and Tyrosine. For researchers who want to better control assay variables, the IMAP Progressive Binding System lets you optimize assay conditions to address key variables such as substrate choice, desirable ATP concentrations and background parameters.
• Evaluation - supports 800 data points in a standard 384-well format • Screening Express - supports 8000 data points in a standard 384 well format • IMAP Purchase Plan (IPP) - configurable annual plan to support multiple high-throughput screens per year
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