iBlot® 7-Minute Blotting System

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iBlot® 7-Minute Blotting System
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The iBlot® 7-Minute Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes, offering the convenience of a bufferless, plug-and-play system.In addition to ease and convenience, the iBlot® system offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample.The iBlot® 7-Minute Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Novex® Midi Gels (8 x 1...Read more

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Reviews

22 Scientists have reviewed this product


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Average Rating: 4.1

Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:25 Feb 2014

  • Application Area:Western Blots

  • iBlot® 7-Minute Blotting System

"I love the iBlot system, used with Blot mini gel tank I can pretty much finish a Western in One day. It would normally take me three days using the old system but I find this very useful! "
Rating: 3.3
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:29 Nov 2013

  • Application Area:Arc Western Blots

  • iBlot® 7-Minute Blotting System

"We have been pleased with the speed and ease of use of this product and will continue to use it in the future."
Rating: 4.3
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:29 Nov 2013

  • Application Area:Muscle Tissue Composition

  • iBlot® 7-Minute Blotting System

"It is a great system. I thoroughly enjoy the practicality and simplicity of the set up. My major issue is the pricing of everything. "
Rating: 2.0
Ease of use
2 out of 5
After sales service
2 out of 5
Value for money
2 out of 5
  • Review date:29 Nov 2013

  • Application Area:Transfer After Western Blotting

  • iBlot® 7-Minute Blotting System

"I find the system not especially useful for transfer of western blot plus not particularly more effective than traditional semi wet or wet transfers. It also requires the purchase of one use reagents which makes the system rather expensive and inconvenient."
Rating: 3.3
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:06 Aug 2013

  • Application Area:Protein expression

  • iBlot® 7-Minute Blotting System

"Very fast but not good for proteins in low quantity. Not good for membrane proteins or for very hydrophobic proteins."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:02 Aug 2013

  • iBlot® 7-Minute Blotting System

"This is a very well made product. It is easy to use and the directions are straight forward. I would recommend this product."
Rating: 4.3
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
3 out of 5
  • Review date:01 Jul 2013

  • Application Area:molecular biology

  • iBlot® 7-Minute Blotting System

"iBlot system is good for low molecular weight protein but not for high molecular weight protein. One needs to standardize case by case if the iBlot system is suitable for use in any particular situation."
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:03 Jun 2013

  • Application Area:cancer cell biology

  • iBlot® 7-Minute Blotting System

"The iBlot system from Invitrogen is time saving. It is a convenient system for transferring your western blot and lets you finish your western blotting in a day-that includes running your gel to visualizing it. My blots always came out clean and its very user friendly. Having said that, it’s a little on the expensive side. Also watch out if you are going for the nitrocellulose membrane. If you are planning on stripping and r- probing go for the PVDF. They are better than the nitrocellulose. Happy Blotting!"
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:03 Jun 2013

  • iBlot® 7-Minute Blotting System

"I started use iBlot@7-minute blotting system 2 years ago. The blotting system is very useful. Really save a lot time. And very efficiently. Before, we usually transfer a membrane for more than 2 hrs, but now, I can finish in 7 minutes. I never worry about not going home on time!"
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:glioma

  • iBlot® 7-Minute Blotting System

"Only buffers purchased from the company will work properly and they don't disclose the compositions of them either. Not all the membranes are compatible."
Rating: 4.3
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:Western Blot

  • iBlot® 7-Minute Blotting System

"The iBlot is a very easy to use system that allows you to blot proteins very quickly.  However, the system requires that you optimize for your protein of interest."
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • iBlot® 7-Minute Blotting System

"This is a very convenient system with which you can save your time for more important things also this system ensures very accurate results as well as easy to use. Thus, I want to recommend this product to my lab colleagues who are novices in research."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:chemical inhibition of ovarian cancer cells

  • iBlot® 7-Minute Blotting System

"iBlot system is efficient and user friendly, it saved us time in western blotting in comparison with the conventional blotting system. This iBlot 7 minute blotting system also gives decent results in the western blot experiments."
Rating: 4.0
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:03 Jun 2013

  • Application Area:cancer

  • iBlot® 7-Minute Blotting System

"This product is easy to use and competitive. We are using this one frequently and it showed reliable data. But the price keeps going up. Even so I would like to continue to use it. Also I would like to find good sources for chemicals which is relatively cheap and has high purity."
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:03 Jun 2013

  • Application Area:Cancer biology

  • iBlot® 7-Minute Blotting System

"It is very easy to use and saves a lot of time. You can start your western gel running in the afternoon and finish before end of the day. Very easy to use and apply on different gels from different company. However it does generate some heat."
Rating: 4.0
Ease of use
5 out of 5
After sales service
2 out of 5
Value for money
5 out of 5
  • Review date:29 May 2013

  • Application Area:western blot

  • iBlot® 7-Minute Blotting System

"I have been doing wet transfers for years; this product is a life saver. Seven minutes and then done. Expensive but well worth the purchase."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:22 May 2013

  • Application Area:Western Blotting

  • iBlot® 7-Minute Blotting System

"After years of 30-180 minute transfers, the iBlot system is a 7-8 minute breeze. The quality of the blot is as good or better than the old technique, but the speed of transfer makes overnight transfers a thing of the past. Some optimization is recommended, even if you think the default is giving great results, try a couple of the others to confirm."
Rating: 4.3
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
5 out of 5
  • Review date:22 May 2013

  • iBlot® 7-Minute Blotting System

"The iBlot is a convenient way to speed up your research. We have been using the iBlot for about 3 years now. Our results using the iBlot were very similar to those with previous methods."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:18 Dec 2012

  • Application Area:Western Blot Transfers

  • iBlot® 7-Minute Blotting System

"The iBlot system is very easy to use and very fast in comparison to traditional transfer methods. It is user-friendly, quick and works well, because of this I would recommend it. However, the only downside is that components of the transfer such as the copper gel and sponge are not reusable, which feels a little bit wasteful."

Description

The iBlot® 7-Minute Blotting System efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply.

A self-contained unit, the iBlot® device uses disposable blotting stacks with integrated nitrocellulose or PVDF membranes, offering the convenience of a bufferless, plug-and-play system.

In addition to ease and convenience, the iBlot® system offers high-efficiency protein transfers for high downstream detection sensitivity—in many cases, higher than wet and semi-dry transfer methods. In the end, you’ll achieve more accurate detection using less sample.

The iBlot® 7-Minute Blotting System is designed to work with multiple polyacrylamide gel sizes, including the midi size, fitting Novex® Midi Gels (8 x 13 cm), E-PAGE™ 48 and 96 gels (13.5 x 10.8 cm), and mini gels (8 x 8 cm).


iBlot® 7-Minute Blotting System Features:

  • Fast - protein transfer in 7 minutes or less
  • High quality transfer - efficient transfers without bubbles or distortions
  • Convenient - Self contained unit without the need for additional buffer or external power supply
  • Reproducible - reduces blot preparation and running variability

Product Overview

Product Image

iBlot® 7-Minute Blotting System

by Life Technologies

4.1/5.0 (22 reviews)
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5






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