Human TNF-alpha/TNFSF1A Quantikine ELISA Kit
See all reviews
Member since 2002
University of Utah- Vascular Lab
Read more »
"The instructions are very clear, and the kit has all the reagents needed to run the assay. The calculations to determine the sample concentrations were a little bit tricky for me, but once I figured it out it was not a problem. The storage recommendations are very important; don't save prepared chemicals longer than recommended or under storage conditions not recommended. The standards curve came out looking really good except for a few times when I neglected to notice the recommended storage conditions for the TNF-alpha stock solution. "
R&D Human TNF-alpha/TNFSF1A Quantikine ELISA Kit - Human TNF-alpha Immunoassay for the quantitative determination of human tumor necrosis factor alpha (TNF-alpha) concentrations in cell culture supernates, serum, and plasma.
The R&D Quantikine Human TNF-alpha Immunoassay is a 3.5 - 4.5 hour solid phase ELISA. This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-alpha has been pre-coated onto a microplate.
R&D Systems Quantikine ELISA Kits are complete and ready-to-use.
Features of the R&D Quantikine ELISA Kits include:
- Complete ready to use kits
- Exhaustively tested for superior quality and reproducibility
- Detailed protocol booklets include inter- and intra-assay precision, recovery, linearity, sensitivity, and sample value data
- Colorimetric detection
R&D Quantikine ELISA Kit Contents:
Kit contents may vary slightly.
Pre-coated 96-well Microplate
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard
- Assay Diluent
- Calibrator Diluent(s)
- Wash Buffer
- Color Reagent A and B
- Stop Solution
- Plate Sealers