Bioneer’s HotStart DNA polymerase uses an exclusive enzyme-mediated Hot Start PCR method that, unlike most other HotStart PCR chemistries, completely releases all polymerase activity during the first denaturation step. Top DNA polymerase is completely inhibited by pyrophosphate at temperatures below 70°C. However, at temperatures above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity.
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Member since: 2011
Organization: University of Colorado