The HiSeq 2000 sequencing system offers unprecedented output and a breakthrough user experience.

Reversible terminator-based sequencing

Leveraging Illumina's proven and widely-adopted, reversible terminator-based sequencing by synthesis chemistry in combination with innovative engineering, HiSeq 2000 delivers the industry's highest sequencing output and fastest data generation rate. Human interaction design features and the easiest sequencing workflow set a new standard for simplicity and user experience.

Sequence at a Scale Never Before Possible

HiSeq 2000 makes it possible for individual labs to take on the largest and most complex sequencing studies at the lowest cost. The ability to process larger numbers of samples and to decode larger and more complex genomes means that virtually any sequencing project is now within reach.

Key features of the Illumina HiSeq 2000 include:

• Performance: The greatest yield of perfect reads and bases greater than Q30

• Throughput: Up to 25 Gb per day for a 2 x 100 bp run.

• Reads: Up to one billion clusters passing filter and up to two billion paired-end reads.

• Speed: Library preparation <6 hours, cluster generation <4 hours (with less than 10 minutes hands-on time), sequencing by synthesis takes just 1.5-8 days and data processing with RTA v1.7. CASAVA v1.7 requires 2 days