Most traditional methods published for isolating hepatocytes use crude and partially purified enzyme preparations including various types of collagenase and other proteases. More recently the use of better characterized preparations of collagenase such as Worthington Types 1 and 4 (CLS-1, 4) have provided better results. All crude collagenase preparations can contain lot-variable contaminating proteases, esterases and other enzymes requiring researchers to pre-screen several lots of enzyme and/or continually modify isolation parameters and protocols.
The Worthington Hepatocyte Isolation System has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells.