Goat IgG TrueBlot™ is a unique horseradish peroxidase conjugated anti-goat IgG immunoblotting (second step) reagent. Goat IgG TrueBlot™ enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/WB data with Goat IgG TrueBlot™, simply substitute the conventional HRP anti-goat IgG blotting reagent with Goat IgG TrueBlot™ and follow the prescribed protocol for sample preparation and immunoblotting. Note that there are three key procedural considerations: 1. Immunoprecipitate should be completely reduced. 2. Milk should be used as the blocking protein for the immunoblot. BSA is not an effective blocker. 3. Protein A or G beads may be used with the mouse, goat and sheep TrueBlot secondaries but not with the rabbit TrueBlot secondary. Use of protein A or G beads with the rabbit TrueBlot will result in contaminating bands.
Feedback
Request info
Write a review
Sharing your experience will help scientists like you. Achieve Reviewer Status and Win an iPad 3 (All reviews published will be entered into the next drawing on May 31st 2013).
