Agilent BioTek Gen5 Software for Detection

The evaluation of neurite outgrowth using high-throughput image-based analysis provides an essential tool for neurobiologists to advance basic, translational, and clinical research in neuroscience. Explore the BioTek Gen5 neurite outgrowth module from Agilent Technologies that offers flexible, automated, multichannel analysis of neurite outgrowth across multiple fluorescence channels and modalities to support a broad range of neurite outgrowth approaches. The module is compatible across Agilent BioTek imaging instruments to provide a single platform solution for neurite outgrowth assays.

Plaque assays remain the standard method for determining viral titers for lysogenic viruses. The assay relies on determining the number of plaque-forming units (PFU) created in a monolayer of virus-infected cells. In this application note, Agilent presents an assay that provides an accurate and efficient method for determining plaque counts and calculating viral titers in a range of microplate densities, including 6-, 24-, 48-, and 96-well formats.

In this application note, BioTek highlights two examples that use a combination upright and inverted automated image acquisition system to demonstrate the ability to rapidly image entire vessel surfaces at low resolution, select only those regions of interest, and capture high resolution images of the ROIs using automated imaging methods.

With BioTek’s Lionheart FX Automated Microscope and Gen5 Image Analysis Software, total cells/volume can be calculated for almost any type of cell, using digitally captured and analyzed images, independent of probes or stains. This process is demonstrated in this free infographic that shows how to quantify pre- and post-fermented yeast cells in corn mash.

As critical components of the innate immune system, macrophages respond to microbes by recognizing molecules, such as the gram-negative bacteria product lipopolysaccharide (LPS), via Toll-like receptors. Receptor activation stimulates a complex signaling network that involves, among others, the NF-κB pathway. Here we quantitate the nuclear translocation of the protein RelA, a component of the NF-κB transcription factor complex, in macrophages using image-based analysis.

3D cell culture has become a well-established in vitro experimental approach as it realistically mimics an in vivo environment. The use of clear U-bottom ultra-low attachment microplates, that minimize cell adherence, has become a standard for applications such as spheroid proliferation. In this application note, BioTek presents data generated with the Cytation™ 5 Cell Imaging Multi-Mode Reader, using PrimeSurface ULA plates to develop simple and robust spheroid assays for brightfield and fluorescence imaging.

In this application note, BioTek demonstrates high-throughput screening and analysis of zebrafish embryos treated with several developmental toxins. Here, BioTek exposes embryos to ethanol, retinoid acid, and cyclopamine, then capture brightfield images of the embryos in multi-well plates with Lionheart™ Automated Microscope. The images are analyzed for size and shape using Gen5™ software. The combination of high-throughput imaging and analysis using a vertebrate model makes this an attractive method for toxicology screening.

Ethanol exposure during development can have devastating effects on the fetus. In this application note, zebrafish are used, an excellent model to study both normal development and toxicity, in order to determine the effects of ethanol exposure during development. Zebrafish embryos were treated with ethanol during the first 24 hours of development and the effects of ethanol treatment on cell death was assessed using acridine orange staining. It was found that exposure of embryos to ethanol results in a dose-dependent increase in cell death overall in the embryo.

Autophagy is critical for the maintenance of cellular homeostasis. However, dysregulated autophagy can lead to death of healthy cells and survival of cancerous cells. Here we describe the use of CYTO-ID® Autophagy Detection Kit in combination with automatedobject-based spot counting to quantitatively assess the effects of starvation and rapamycin on cellular autophagy by determining the size and number of autophagosomes per cell.

To meet the demand for increased hepatotoxicity testing, automation has been incorporated to streamline the procedure and reduce the need for large-scale manual manipulations. This application note describes how to combine liquid handling with a novel cell imaging multi-mode reader to perform automated 3D hepatotoxicity studies.

Watch this video to discover how BioTek Instruments, Inc. could enhance your cell imaging workflow. Learn how the Cytation™ 5 Cell Imaging Multi-Mode Reader, combined with Gen™ 5 Data Collection & Analysis Software and the BioSpa™ 8 Automated Incubator facilitate live cell imaging automation, with versatile assay detection, optional imaging modes and environmental controls for long-term imaging.

New Peltier cooling module by BioTek prevents temperature fluctuations in multimode cell imagers

AutoScratch tool to facilitate 2D collective cell migration and invasion applications

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Capturing live cell images and transforming them into intelligent information

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