FuGENE 6 Transfection Reagent

FuGENE 6 Transfection Reagent
by Roche Applied Science - a member of the Roche Group
 3.8/5.0 (16 reviews)

Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratorie...read more

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Reviews

AVERAGE RATING: 3.8

16 scientists have reviewed this product

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  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY

Sheri Hiroi


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Member since 2013

Organization:
Amazona State University
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RATING: 2.3

  • 2 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 02 Dec 2013

Application Area: Transfecting Agent
FuGENE 6 Transfection Reagent

"Although fugene 6 was ok to use it was discontinued and I had to switch to another reagent in middle of my study. The problem with fugene and other transfecting reagent is that it evaporates very easily and the container that it comes in is not made appropriately to protect it from evaporation. Also you can’t aliquote because of this same issue."

Read 16 reviews

Joonbae Seo


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Member since 2013

Organization:
Iowa State University
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: Plasmids Transfection
FuGENE 6 Transfection Reagent

"I have used fuGENE 6 for my transfection of plasmids. Their protocol is easy to use. A good point is that it is less toxic in the cell."

Chia-Yi Tseng


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Member since 2013

Organization:
Chung Yuan Christian University
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RATING: 3.0

  • 4 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: neuro2A Cell Line
FuGENE 6 Transfection Reagent

"It is working well in the cell line but not well in the primary neuronal culture. Especially for the div over 12. "

Ricardo Marquez Gomez


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Member since 2013

Organization:
Cinvestav
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RATING: 4.3

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: Adenosine And Dopamine GPCRs Expression In Cell Culture
FuGENE 6 Transfection Reagent

"We used this to utilize lipofectamine to make stable transfection and transient transfections. We recently acquired FuGene and since then it has been easier and more reliable to make the transfections needed."

Ghania Ait-Ghezala


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Member since 2013

Organization:
The Roskamp Institute
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RATING: 3.0

  • 4 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 29 Nov 2013

Application Area: Neuronal Cells
FuGENE 6 Transfection Reagent

"This works very well for neuronal cells transfection as well as an electroporation. It is easy use, quick and works well for primary cells."

Susan Wong


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Member since 2013

Organization:
University at Buffalo, The State University of New York
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RATING: 4.0

  • 3 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 28 Nov 2013

Application Area: Transfection Of Primary Neuronal Culture
FuGENE 6 Transfection Reagent

"I really liked it and thought it was very effective. The instructions were very clear and the protocol was easy to follow."

Claudia Conte


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Member since 2012

Organization:
University Of Dundee
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RATING: 2.3

  • 3 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 16 Dec 2012

FuGENE 6 Transfection Reagent

"It is a good reagent to work with in general. With some cell lines (U2OS) I have found it not working as well as in others (such as HeLa)."

LAUREN ZASADIL


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Member since 2010

Organization:
UNIV. OF WISCONSIN-MADISON
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RATING: 4.0

  • 4 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 11 Jun 2012

Application Area: Transfection of cell culture for stable protein expression.
FuGENE 6 Transfection Reagent

"This product did work but the transfection efficiency was lower than I would have liked."

DANIEL ANDRADE


Status:


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Member since 2010

Organization:
UNIV. OF UTAH HUNTSMAN CANCER INST.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 06 Jun 2012

Application Area: Cell transfections
FuGENE 6 Transfection Reagent

"Very good levels of transfections. Only requires a small volume- up to 6ul for a 10ml plate of 293 cells. Good for making retrovirus on 293 cells."

Carl Hurt


Status:


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Member since 2011

Organization:
Standford University
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RATING: 2.6

  • 4 out of 5EASE OF USE
  • 2 out of 5AFTER SALES SERVICE
  • 2 out of 5VALUE FOR MONEY




Review date: 07 Dec 2011

FuGENE 6 Transfection Reagent

"Limited success, low transfection rate. Decreased transfection rate with continued cell passage."

LAKSHMI UPPALAPATI


Status:


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Member since 2011

Organization:
KANSAS STATE UNIV.
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 13 Jul 2011

FuGENE 6 Transfection Reagent

"Exceptionally good to transfect the cells which are usually very tough to get transfected."

KAREN VRIJENS


Status:


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Member since 2011

Organization:
ST. JUDE CHILDREN'S RES. HOSP.
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 11 Jul 2011

FuGENE 6 Transfection Reagent

"Easy to use transfection reagent. Company has very knowledgeable staff for after-sale consultation."

Jill Larsen


Status:


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Member since 2009

Organization:
UT SOUTHWESTERN MEDICAL CENTER
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RATING: 4.0

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 08 Jul 2011

FuGENE 6 Transfection Reagent

"Great transfection lipid as works well with most lines."

Lindsay Mccullough


Status:


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Member since 2010

Organization:
Florida Atlantic Univ
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RATING: 4.3

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 21 Jan 2011

FuGENE 6 Transfection Reagent

"Works better than other transfection reagents."

Bin Wu


Status:


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Member since 2010

Organization:
Albert Einstein College Of Medicine
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RATING: 2.3

  • 3 out of 5EASE OF USE
  • 1 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 05 Jan 2011

FuGENE 6 Transfection Reagent

Mohammed Akbar


Status:


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Member since 2010

Organization:
Niaaa/ Nih
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 19 Jan 2010

FuGENE 6 Transfection Reagent

Description

 

Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world.

With a mention in more than 8,500 publications, Roche FuGENE 6 Transfection Reagent has become one of the most widely used transfection reagents in the world. A list of more than 700 successfully transfected cell types, including many "hard-to-transfect" cell lines transfected with DNA plasmids (including very large plasmids >12 kb), is available in our Transfected Cells Database. To consult this database and to find more information about the use of FuGENE® 6 Transfection Reagent, please visit our Transfection Special Interest Site.

Features of the Roche FuGENE 6 Transfection Reagent:

  • Avoid time-consuming optimization procedures by relying on high transfection efficiency proven in more than 700 cell types, including many "hard-to-transfect" cells.
  • Generate physiologically relevant results with the low-cytotoxicity of FuGENE® 6 Transfection Reagent. A recent gene-chip study demonstrated that a leading competitor´s transfection reagent significantly influenced the expression of more than 2,000 genes, compared to only 30 genes affected by FuGENE® 6 Transfection Reagent.
  • Transfect in serum-free or serum-containing media. FuGENE® 6 Transfection Reagent is highly efficient both in the presence and absence of serum. In most cases, the same cell culture medium used for routine cell culture (e.g., one that contains serum) can also be used during transfection. This is especially useful in cases where transfection efficiency improves in the presence of serum.
  • Save time and effort by eliminating multiple handling steps. Simply dilute FuGENE® 6 Transfection Reagent, incubate it with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Use less reagent per transfection. Perform more transfections with 1 ml of FuGENE® 6 Transfection Reagent than with 1 ml of competitors’ reagents.
  • Obtain a higher yield of stable transfections through higher cell-survival rates.

Product Overview

FuGENE 6 Transfection Reagent by Roche Applied Science - a member of the Roche Group
FuGENE 6 Transfection Reagent




  • 4out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY





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