- Ease of use
- After sales service
- Value for money
Description
Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world.
With a mention in more than 8,500 publications, Roche FuGENE 6 Transfection Reagent has become one of the most widely used transfection reagents in the world. A list of more than 700 successfully transfected cell types, including many "hard-to-transfect" cell lines transfected with DNA plasmids (including very large plasmids >12 kb), is available in our Transfected Cells Database. To consult this database and to find more information about the use of FuGENE® 6 Transfection Reagent, please visit our Transfection Special Interest Site.
Features of the Roche FuGENE 6 Transfection Reagent:
- Avoid time-consuming optimization procedures by relying on high transfection efficiency proven in more than 700 cell types, including many "hard-to-transfect" cells.
- Generate physiologically relevant results with the low-cytotoxicity of FuGENE® 6 Transfection Reagent. A recent gene-chip study demonstrated that a leading competitor´s transfection reagent significantly influenced the expression of more than 2,000 genes, compared to only 30 genes affected by FuGENE® 6 Transfection Reagent.
- Transfect in serum-free or serum-containing media. FuGENE® 6 Transfection Reagent is highly efficient both in the presence and absence of serum. In most cases, the same cell culture medium used for routine cell culture (e.g., one that contains serum) can also be used during transfection. This is especially useful in cases where transfection efficiency improves in the presence of serum.
- Save time and effort by eliminating multiple handling steps. Simply dilute FuGENE® 6 Transfection Reagent, incubate it with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
- Use less reagent per transfection. Perform more transfections with 1 ml of FuGENE® 6 Transfection Reagent than with 1 ml of competitors’ reagents.
- Obtain a higher yield of stable transfections through higher cell-survival rates.
Reviews
Chris Provost Status: See all reviews Member since 2012 Organization: New England Biolabs Read more » | RATING: 3.7 
"FuGene 6 is an easy to use product that gives good results. It's a little bit pricey, but the results are generally worth it."   |
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| Claudia Conte Status: See all reviews Member since 2012 Organization: University Of Dundee Read more » | RATING: 2.3
"It is a good reagent to work with in general. With some cell lines (U2OS) I have found it not working as well as in others (such as HeLa)." |
| LAUREN ZASADIL Status: See all reviews Member since 2010 Organization: UNIV. OF WISCONSIN-MADISON Read more » | RATING: 4.0
"This product did work but the transfection efficiency was lower than I would have liked." |
| DANIEL ANDRADE Status: See all reviews Member since 2010 Organization: UNIV. OF UTAH HUNTSMAN CANCER INST. Read more » | RATING: 5.0
"Very good levels of transfections. Only requires a small volume- up to 6ul for a 10ml plate of 293 cells. Good for making retrovirus on 293 cells." |
| Carl Hurt Status: See all reviews Member since 2011 Organization: Standford University Read more » | RATING: 2.6
"Limited success, low transfection rate. Decreased transfection rate with continued cell passage." |
| LAKSHMI UPPALAPATI Status: See all reviews Member since 2011 Organization: KANSAS STATE UNIV. Read more » | RATING: 5.0
"Exceptionally good to transfect the cells which are usually very tough to get transfected." |
| KAREN VRIJENS Status: See all reviews Member since 2011 Organization: ST. JUDE CHILDREN'S RES. HOSP. Read more » | RATING: 4.3
"Easy to use transfection reagent. Company has very knowledgeable staff for after-sale consultation." |
| Jill Larsen Status: See all reviews Member since 2009 Organization: UT SOUTHWESTERN MEDICAL CENTER Read more » | RATING: 4.0
"Great transfection lipid as works well with most lines." |
| Lindsay Mccullough Status: See all reviews Member since 2010 Organization: Florida Atlantic Univ Read more » | RATING: 4.3
"Works better than other transfection reagents." |
| Bin Wu Status: See all reviews Member since 2010 Organization: Albert Einstein College Of Medicine Read more » | RATING: 2.3
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| Mohammed Akbar Status: See all reviews Member since 2010 Organization: Niaaa/ Nih Read more » | RATING: 5.0
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