FuGENE 6 Transfection Reagent

3.8
/
5.0
  |  16 reviews


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Average Rating: 3.8
16 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 2.3

  • Application Area: Transfecting Agent

"Although fugene 6 was ok to use it was discontinued and I had to switch to another reagent in middle of my study. The problem with fugene and other transfecting reagent is that it evaporates very easily and the container that it comes in is not made appropriately to protect it from evaporation. Also you can’t aliquote because of this same issue."

Review date: 02 Dec 2013 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Iowa State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Plasmids Transfection

"I have used fuGENE 6 for my transfection of plasmids. Their protocol is easy to use. A good point is that it is less toxic in the cell."

Review date: 29 Nov 2013 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Chung Yuan Christian University



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    2 out of 5
Rating: 3.0

  • Application Area:neuro2A Cell Line

"It is working well in the cell line but not well in the primary neuronal culture. Especially for the div over 12. "

Review date: 29 Nov 2013 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Cinvestav



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:Adenosine And Dopamine GPCRs Expression In Cell Culture

"We used this to utilize lipofectamine to make stable transfection and transient transfections. We recently acquired FuGene and since then it has been easier and more reliable to make the transfections needed."

Review date: 29 Nov 2013 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: The Roskamp Institute



  • Ease of use
    4 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Rating: 3.0

  • Application Area:Neuronal Cells

"This works very well for neuronal cells transfection as well as an electroporation. It is easy use, quick and works well for primary cells."

Review date: 29 Nov 2013 | FuGENE 6 Transfection Reagent


  • Status:

    Reviewer

  • Member since: 2013

  • Organization: University at Buffalo, The State University of New York



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Transfection Of Primary Neuronal Culture

"I really liked it and thought it was very effective. The instructions were very clear and the protocol was easy to follow."

Review date: 28 Nov 2013 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: University Of Dundee



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    2 out of 5
Rating: 2.3
"It is a good reagent to work with in general. With some cell lines (U2OS) I have found it not working as well as in others (such as HeLa)."

Review date: 16 Dec 2012 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: UNIV. OF WISCONSIN-MADISON



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Rating: 4.0

  • Application Area:Transfection of cell culture for stable protein expression.

"This product did work but the transfection efficiency was lower than I would have liked."

Review date: 11 Jun 2012 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: UNIV. OF UTAH HUNTSMAN CANCER INST.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

  • Application Area:Cell transfections

"Very good levels of transfections. Only requires a small volume- up to 6ul for a 10ml plate of 293 cells. Good for making retrovirus on 293 cells."

Review date: 06 Jun 2012 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Standford University



  • Ease of use
    4 out of 5
    After sales service
    2 out of 5
    Value for money
    2 out of 5
Rating: 2.7
"Limited success, low transfection rate. Decreased transfection rate with continued cell passage."

Review date: 07 Dec 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: KANSAS STATE UNIV.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0
"Exceptionally good to transfect the cells which are usually very tough to get transfected."

Review date: 13 Jul 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: ST. JUDE CHILDREN'S RES. HOSP.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Easy to use transfection reagent. Company has very knowledgeable staff for after-sale consultation."

Review date: 11 Jul 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2009

  • Organization: UT SOUTHWESTERN MEDICAL CENTER



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Rating: 4.0
"Great transfection lipid as works well with most lines."

Review date: 08 Jul 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Florida Atlantic Univ



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3
"Works better than other transfection reagents."

Review date: 21 Jan 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Albert Einstein College Of Medicine



  • Ease of use
    3 out of 5
    After sales service
    1 out of 5
    Value for money
    3 out of 5
Rating: 2.3

Review date: 05 Jan 2011 | FuGENE 6 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Niaaa/ Nih



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rating: 5.0

Review date: 19 Jan 2010 | FuGENE 6 Transfection Reagent

Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world.

With a mention in more than 8,500 publications, Roche FuGENE 6 Transfection Reagent has become one of the most widely used transfection reagents in the world. A list of more than 700 successfully transfected cell types, including many "hard-to-transfect" cell lines transfected with DNA plasmids (including very large plasmids >12 kb), is available in our Transfected Cells Database. To consult this database and to find more information about the use of FuGENE® 6 Transfection Reagent, please visit our Transfection Special Interest Site.

Features of the Roche FuGENE 6 Transfection Reagent:

  • Avoid time-consuming optimization procedures by relying on high transfection efficiency proven in more than 700 cell types, including many "hard-to-transfect" cells.
  • Generate physiologically relevant results with the low-cytotoxicity of FuGENE® 6 Transfection Reagent. A recent gene-chip study demonstrated that a leading competitor´s transfection reagent significantly influenced the expression of more than 2,000 genes, compared to only 30 genes affected by FuGENE® 6 Transfection Reagent.
  • Transfect in serum-free or serum-containing media. FuGENE® 6 Transfection Reagent is highly efficient both in the presence and absence of serum. In most cases, the same cell culture medium used for routine cell culture (e.g., one that contains serum) can also be used during transfection. This is especially useful in cases where transfection efficiency improves in the presence of serum.
  • Save time and effort by eliminating multiple handling steps. Simply dilute FuGENE® 6 Transfection Reagent, incubate it with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Use less reagent per transfection. Perform more transfections with 1 ml of FuGENE® 6 Transfection Reagent than with 1 ml of competitors’ reagents.
  • Obtain a higher yield of stable transfections through higher cell-survival rates.