Millipore’s The FlowCellect™ Human Embryonic Stem Cell Surface Marker Characterization kit is designed to facilitate an easier and quicker way to track surface marker expression of TRA-1-60, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both TRA-1-60 and SSEA4 and not SSEA1. This quick test will allow researchers to determine the quality of their cells in culture as well as see changes in marker expression during a differentiation protocol.
Sufficient reagents are provided for 25 3-color samples and 5 isotype control tests. The kit includes all optimized fluorescently labeled antibodies and buffers necessary for analysis. To verify antibody specificity, all antibodies were tested against the ENSTEM-A cell line that does not express TRA-1-60, SSEA4 or SSEA1. A cell strainer is also included to ensure samples are devoid of large cell clumps that can alter cell analysis. Detailed assay instructions are also included to assist in analysis and to ensure that the correct cell concentration is obtained for flow cytometric analysis.