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"Very easy to use kit, taking cells from 96 well plate straight through to Ct qRT-PCR analysis. Allows for fast extraction to final analysis within the hour."
FastLane Cell cDNA Kit. For high-speed preparation of cDNA without RNA purification for use in real-time RT-PCR. Kit contents: Buffer FCW, Buffer FCP, and components for 50 x 20 l reverse-transcription reactions (gDNA Wipeout Buffer, Quantiscript Reverse Transcriptase, Quantiscript RT Buffer, RT Primer Mix, and RNase-Free Water).
Benefits: From cells to cDNA in only 4 steps and under 45 minutes. Sensitive detection of even low-abundance transcripts. Easy parallel processing of several samples. Integrated gDNA removal allows detection of RNA only. No need to design RNA-specific primers or probes