Fab Fragment Affinity-Purified Secondary Antibodies
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. Block endogenous immunoglobulins on cells, tissues , or other surfaces. Block exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. Fab-label primary antibodies in solution without compromising activity.
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Works well at high concentrations
Immunohistochemistry
Very useful for an immunohistochemistry with two primary antibodies from the same host. It covers the first primary antibody, presenting it as a different species. Used at a concentration of 40 ug/ml with the following protocol: https://www.jacksonimmuno.com/technical/products/protocols/double-labeling-same-species-primary/example-b
Review Date: 8 Aug 2016 | Jackson ImmunoResearch Laboratories Inc.
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region.
- Block endogenous immunoglobulins on cells, tissues , or other surfaces.
- Block exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species.
- Fab-label primary antibodies in solution without compromising activity.
Guide to Multiple Labeling with Secondary Antibodies
Jackson ImmunoResearch provides secondary antibodies with minimal cross-reactivity for multiple labelling. This poster provides an example of a multiple-labelling protocol, using Jackson ImmunoResearch reagents. If you require a hard copy of the poster, please email marketing@jacksonimmuno.com.
Western Blot Troubleshooting Guide
Western blotting is a staple technique of the molecular biology lab. The robust nature of the antigen-antibody interaction allows the presence of specific proteins and peptides to be detected from complex mixtures. If the Western blot is not behaving as expected, the following troubleshooting guide may help isolate the problem.
Mouse on mouse labeling with Fab fragment blocking
This video by Jackson ImmunoResearch Laboratories demonstrates how to use Fab fragments to successfully label primary antibodies from the same species as the tissue sample (e.g. mouse on mouse).
MicroRNA Downregulates Proteins Implicated in Chronic Pain
Dr Maria Lopez deciphers microRNA regulation in pain perception. Read on to learn how she successfully uses multiple primary antibodies from the same host species.
