DRAQ5

The proven far-red probe for live or fixed cells, spectrally compatible with UV / Vis-range fluors.

DRAQ5™ is a novel far-red fluorescent LIVE cell DNA dye that can be used in combination with other common fluors, especially GFP fusions & FITC-tags. DRAQ5™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms. Biostatus is happy to provide extensive pre-sales technical support for your application.Main application areas are HCS, flow cytometry and fluorescence microscopy.* Rapid staining of dsDNA/nuclei of LIVE or fixed cells * Easy to use - no lyse, no wash, no RNase needed * Ideal for use with GFP & FITC labels - DRAQ5™ fluoresces in the far-red region * Cost-effective analysis FOR HCS LABS:DRAQ5™ is the ideal nuclear counterstain for HCS.  It can reduce the screening time by 50%. It permits simultaneous visualisation ...Read more

Receive your quote directly from Biostatus Limited.

Reviews

47 Scientists have reviewed this product


Write your own review

Average Rating: 4.7

Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:04 Aug 2015

  • Application Area:Analyze cell viability and nuclei morphology

  • DRAQ5

"Easy to use and sensitive. It’s a good dye to complete flow cytometry, confocal microscopy or multispectral flow cytometry evaluations. "
Rating: 3.3
Ease of use
4 out of 5
After sales service
3 out of 5
Value for money
3 out of 5
  • Review date:04 Aug 2015

  • DRAQ5

"We use DRAQ5 from many years both in human and mouse samples formalin fixed or in OCT with good results. I would recommend this product. It is not expensive and the results are good."
Rating: 5.0
We prefer to use DRAQ5 as a far-red nuclear stain for most of our confocal microscopy experiments involving gene transfection studies with a variety of nanoparticles.
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:22 Jun 2015

  • Application Area:We use DRAQ5 as a nuclear stain for imaging in a variety of cellular drug delivery experiments.

  • DRAQ5

"We actually prefer to use DRAQ5 as a far-red nuclear stain for most of our confocal microscopy experiments involving gene transfection studies with a variety of nanoparticles including lipid-based, peptide-based NPs and carbon nanotubes. We obtained excellent results in a variety of cell types in live cell assays and also in cryostat sectioned skin and eye tissues. We highly appreciate the prompt shipment and outstanding scientific support."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:19 Jun 2015

  • Application Area:Co-stainings for various mammalian cell lines and tissues (cryo- and paraffin sections)

  • DRAQ5

"DRAQ5 is easy to use and gives highly reproducible results in various mammalian cell lines as well as tissue sections."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:17 Jun 2015

  • Application Area:microscopy imaging

  • DRAQ5

"We used DRAQ5 for nuclear counterstaining in filamentous fungi for imaging. DRAQ5 works perfectly using multiple fluorescent markers, since its far red emission avoids signals in other frequently used channels and gives bright signals. It is easy to use and ensures a long lasting fluorescence."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:17 Jun 2015

  • Application Area:To stain DNA in fixed human peripheral blood lymphocytes

  • DRAQ5

"DRAQ 5 is an excellent product that is easy to use. I would recommend it to anyone wishing to stain DNA in live or fixed cells. One major benefit over other nuclear stains is that excess dye does not need to be washed out as is the case with many other DNA stains. We did not have any discussion with the company after purchase simply because none was necessary. "
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:16 Jun 2015

  • Application Area:Immunhistochemistry on frozen sections

  • DRAQ5

"Easy to use (diluted in phosphate buffer; dilution factor can be increased if increasing incubation time ), sharp staining and contrast, extremely stable compared to other long-red nuclear counterstains we have tried in the lab for immunohistology (no need of special anti-fade mounting medium)."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:15 May 2015

  • Application Area:for in cell western and total protein normalization

  • DRAQ5

"Quite an easy to use product that you could use for total protein cell normalization during in cell western analysis."
Rating: 4.0
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
3 out of 5
  • Review date:30 Apr 2015

  • Application Area:Slices of fixed tissue

  • DRAQ5

"DRAQ5 is a great alternative to label cell nucleus when you have other different fluorescent markers in the same sample. Its far red emission makes it compatible to other fluorophores of the spectra without crossing signals."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:10 Apr 2015

  • Application Area:Fixed-cell staining of adherent cells

  • DRAQ5

"DRAQ5 is easy-to-use and stains cells brightly. Great alternative when using multiple markers or if a stain has a large spectrum."
Rating: 3.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
2 out of 5
  • Review date:06 Mar 2015

  • Application Area:High Content imaging, cell based assays

  • DRAQ5

"DRAQ5 is our most established nuclear stain for cell based assays. It also stains the cytoplasm to some degree which is useful for cytoplasm detection. It's emission in the far red is useful as it does not cause cross signals on lower wavelength-excited channels (while Dapi or Hoechst do)! It is quite expensive though! After sales care not required. "
Rating: 4.3
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
3 out of 5
  • Review date:26 Feb 2015

  • Application Area:For In-Cell Westerns on the Odyssey Imager

  • DRAQ5

"Using DRAQ5 on the Odyssey to approximate cell numbers is not as sensitive as manual counts of nuclei on a microscope but is much faster and less biased."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:23 Feb 2015

  • Application Area:for nucleus staining

  • DRAQ5

"I use this often when I use microscope without filter for 350nm wave length. It is easy to use and stains very well. I always recommend this whenever there are people who want to use nucleus staining."
Rating: 4.0
Ease of use
5 out of 5
After sales service
3 out of 5
Value for money
4 out of 5
  • Review date:23 Feb 2015

  • Application Area:Image stream, confocal microscopy

  • DRAQ5

"We mainly use DRAQ5 as a nuclear stain for Image stream (cells in suspension). It is easy to use, and very bright."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:05 Feb 2015

  • Application Area:Use to stain nuclei for imaging flow cytometry

  • DRAQ5

"DRAQ5 is incredibly easy to use. Just dilute and add to cells immediately before acquiring days. Dye incorporates quickly into both live and fixed cells. It also gives a nice bright stain of the nucleus that has crisp edges. Post-sales, the company was always readily available for any questions or to help with designing experiments with any of their other products. DRAQ5 is excitement stable, even with left diluted at room temperature and very little dye is needed to stain cells."
Rating: 4.3
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
4 out of 5
  • Review date:30 Jan 2015

  • Application Area:We used DRAQ5 in immunofluorescence studies.

  • DRAQ5

"DRAQ5 enable us to efficiently stain the nuclei of our mammalian cells (prostate cancer and neuronal cell lines). We used it in a mix with our secondary antibodies."
Rating: 4.7
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money
5 out of 5
  • Review date:30 Jan 2015

  • Application Area:We use DRAQ5 in On- and In-cell-Western assays to account for wll to well variations in cell numbers

  • DRAQ5

"Overall DRAQ5 is an excellent product with simple and easy to use instructions. The dye has very good sensitivity and specificity and can be easily plugged into any protocol with minimal change. DRAQ5 has cut down on protocol time and the high concentration of the stock solution allows one to conduct multiple studies with one vial of solution; thus dramatically reducing costs and time compared to similar nuclear dyes. "
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:16 Jan 2015

  • Application Area:Light microscopy and flow cytometry

  • DRAQ5

"We need to counterstain for DNA in most of our experiments using human cells. DRAQ5 is rapid, simple and very efficient and, very importantly, requires a far-red excitation, much more common and less toxic for live cells than UV lasers (required for DAPI/Hoechst staining). "
Rating: 4.7
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
4 out of 5
  • Review date:08 Jan 2015

  • Application Area:Live cell nuclear detection

  • DRAQ5

"We use DRAQ5 for high content screening in live HEK293 cells for the purpose of nuclear detection and segmentation. The dye very simple to use and works perfectly at very low concentrations in HEK293 cells with very low toxicity."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:02 Dec 2014

  • Application Area:We use DRAQ5 on the Amnis Imagestream to visualize nuclear translocation of transcription

  • DRAQ5

"We have used DRAQ5 for Amnis ImageStream analysis for several years now and it has a nice consistently bright staining. We found this dye to be more reliable and less problematic (bleeding into subsequent samples, contaminating tubes, compensation issues) than various other dyes such as Dapi and hoechst dyes. We add 2-3ul of a 1:1000 dilution to our cells (1-2x10^6)/100ul just before acquisition. Officially our vial has expired (for at least a year), but we have seen no reduction in signal. We have used DRAQ5 for traditional flow-cytometry as well, to either eliminate or select cell-doublets or 2-cell conjugates. For flow we use a 10-fold higher concentration than for imaging, which makes it less cost-effective than some other live dyes in this setting."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:28 Oct 2014

  • Application Area:Used as a nucleus stain

  • DRAQ5

"Very effective in imaging, minimum interference with our emission wavelength we study. Easy to use, just minutes of incubation, no unnecessary waiting. I would highly recommend this product to fellow researchers in similar fields."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:27 Oct 2014

  • Application Area:IHC of fixed mouse spinal cord tissue sections

  • DRAQ5

"This dye nicely stains the nuclei even in 60 micron thick spinal cord tissue sections in just 10 minutes. It helped us quickly visualize the aggregation of leukocyte infiltrates across the spinal cord vessels in a mouse model of Multiple Sclerosis."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:27 Oct 2014

  • Application Area:We are using DRAQ5 to look for infiltrating cells into the CNS parenchyma during neuroinflammation.

  • DRAQ5

"This product is easy to use, very efficient and effective. It always has good staining results. "
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:10 Sep 2014

  • Application Area:In-Cell Western

  • DRAQ5

"Our lab routinely uses DRAQ5 for cells normalisation using the In-Cell Western Technique. Overall, we are satisfied, the product works well, it is easy to use and it works very well with the Odyssey Classic - Detection System based on Infrared Technology."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:10 Sep 2014

  • Application Area:CELLULAR BIOLOGY

  • DRAQ5

"DRAQ5 is a trouble-free and versatile reagent to be used in immunofluorescence analysis. It is a high quality product. "
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:18 Aug 2014

  • Application Area:biomedicine

  • DRAQ5

"This is easy to use with high imaging resolution and is good value for money."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:05 Aug 2014

  • Application Area:cell biology, immunology, receptor-signaling research

  • DRAQ5

"This product is very useful for us in intracellular staining of receptors for localization. It is an easy to use nuclear counter stain because of its advantageous spectral properties (it does not cover at least two or three out of the available visible range optical channels in confocal microscopy, like propidium iodide). Sometimes we can also utilize its unique property that it does not need cell permeabilization that other nucleic acid stains require."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:31 Jul 2014

  • Application Area:Fixed granulocyte DNA staining

  • DRAQ5

"Good signal with no detectable bleed through in other fluorescence channels. Quick staining protocol, superior to other DNA dyes we’ve tested."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:15 Jul 2014

  • Application Area:Bio-nanotechnology

  • DRAQ5

"It is very easy to use, and very versatile. It gives good signal without photo-bleaching. I used Draq5 for both fixed and live cell imaging. I recommended to other researchers who is involves in live cell imaging and those working on drug delivery."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:14 Jul 2014

  • Application Area:Immunohistochemistry

  • DRAQ5

"I have been doing immunohistochemistry for many years and found that DRAQ5 nuclear stain is far better than other nuclear stains as it provides better resolution and staining results."
Rating: 5.0
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5
  • Review date:06 Jun 2014

  • Application Area:Molecular Medicine

  • DRAQ5

"DRAQ5 is easy to use. In my research, DRAQ5 was used to determine cellular DNA sets immediately after fluorescence measurement of cellular photosensitizer, giving quantitive analysis of drug uptake. The quality of experiment was excellent."

Description

DRAQ5™ is a novel far-red fluorescent LIVE cell DNA dye that can be used in combination with other common fluors, especially GFP fusions & FITC-tags.

DRAQ5™ has many applications and is highly compatible with existing protocols across a wide range of instrumentation platforms. Biostatus is happy to provide extensive pre-sales technical support for your application.

Main application areas are HCS, flow cytometry and fluorescence microscopy.

* Rapid staining of dsDNA/nuclei of LIVE or fixed cells
* Easy to use - no lyse, no wash, no RNase needed
* Ideal for use with GFP & FITC labels - DRAQ5™ fluoresces in the far-red region
* Cost-effective analysis

FOR HCS LABS:
DRAQ5™
is the ideal nuclear counterstain for HCS. 
It can reduce the screening time by 50%.
It permits simultaneous visualisation of the cytoplasm and the nucleus with one fluorophore!!! 
Suitable for all HCS platforms including GE InCell 3000 and Evotec Technologies' Opera

Product Overview

Product Image

DRAQ5

by Biostatus Limited | Available Worldwide

4.7/5.0 (47 reviews)
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
5 out of 5






Receive your quote directly from Biostatus Limited for DRAQ5