Direct-zol™ RNA MiniPrep

Direct-zol™ RNA MiniPrep
 4.7/5.0 (2 reviews)

Zymo Research

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including ...read more

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Zymo Research

Reviews

John Corthell


Status:


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Member since 2012

Organization:
University of Michigan
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RATING: 4.7

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 20 Dec 2013

Application Area: Neuroscience
Direct-zol™ RNA MiniPrep

"This kit is easy to use and has all the advantages of phenol: chloroform extraction with much less hassle. The RNA extraction with this kit takes less than 5 minutes, either directly from the Direct-zol(TM) itself or from the chloroform step. If you take the RNA from the chloroform extract, then you can still extract the protein and DNA. Furthermore, the RNA is high quality and gives good spectrophotometer readings (the 260/280 and 260/230 readings which are typically close to 2.0). This kit is cheap and works well. I highly recommend this for anyone who is looking to extract RNA and continue to use their phenol: chloroform extraction reagents."

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Philippe Demougin


Status:


See all reviews
Member since 2003

Job Title:
Research Scientist / Senior / PI
Discipline:
Molecular biology
Work Field:
Academia / University
Organization:
Biozenrum
Read more »
RATING: 4.7

  • 5 out of 5EASE OF USE
  • 4 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 14 Jan 2013

Application Area: Molecular Biology
Direct-zol™ RNA MiniPrep

"My experience with this kit is very positive. The trizol lysate is directly loaded onto the column without prior phase separation. Also, the small RNA are preserved (co-isolated) with total RNA even when DNAse I is performed. However, the only drawback is you need to make sure you don't over saturate the column."

Description

 

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product.

The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

• The entire procedure typically takes about 5-10 minutes
• Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar
• Bypasses phase separation and precipitation procedures
• DNase I included for DNA-free RNA

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