- Ease of use
- After sales service
- Value for money
Description
The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product.
The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
• The entire procedure typically takes about 5-10 minutes
• Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar
• Bypasses phase separation and precipitation procedures
• DNase I included for DNA-free RNA
Reviews
Philippe Demougin Status: See all reviews Member since 2003 Job Title: Research Scientist / Senior / PI Discipline: Molecular biology Work Field: Academia / University Organization: Biozenrum Read more » | RATING: 4.7 
"My experience with this kit is very positive. The trizol lysate is directly loaded onto the column without prior phase separation. Also, the small RNA are preserved (co-isolated) with total RNA even when DNAse I is performed. However, the only drawback is you need to make sure you don't over saturate the column."   |
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