Direct-zol™ RNA MiniPrep

Manufacturer Zymo Research  |   Model: R2052
4.7
/
5.0
  |  66 reviews
The Direct-zol™ RNA Kits provide a streamlined method for the purification of up to 100 ug (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Spin column procedure bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.


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Average Rating: 4.7
66 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


My go-to RNA kit for qPRC and RNA-seq
Rating: 5.0

  • Application Area: Isolating RNA from cells.

"Works great, less steps than other kits so the process takes less time. Quality RNA. Love the additional optional DNAse treatment as well."

Review date: 08 Aug 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Centro de investigación en alimentación y desarrollo A.C.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, very practical
Rating: 5.0

  • Application Area:Analyze antigen variability

"It is very easy to use, rapid and it gives you high quality RNA material, good for cloning and sequencing. However, I have low yield of RNA eluted, so I just repeated the last step."

Review date: 08 Aug 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Universidad de San Martin



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    4 out of 5
Excellent results, easy to use
Rating: 3.7

  • Application Area:Gene expression

"This product allows RNA isolation from TRIzol homogenates without chloroform extraction. Also, microRNA can be isolated."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Tulane University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great kit, will keep using!
Rating: 5.0

  • Application Area:Extract RNA from mice brain regions

"The Directzol Miniprep Kit from Zymo was incredibly easy to use with several fewer steps compared to the mirVana RNA extraction kit. The quality of extracted RNA is excellent and within the same concentrations as other kits used. In addition to the kit being easier to use the cost was also lower than other kits our lab has used."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NARIC ABC



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Fast, effective, value for money
Rating: 4.3

  • Application Area:RNA purification for expression studies from Medicago and relative roots and root nodules

"Makes the RNA prep much easier, faster direct from TRIzol. I can ask for help the sale company. Got good RNA preps with the kit - I would prefer even more active DNAse for on column genomic DNA digestion."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep


  • Status:

    Reviewer

  • Member since: 2016

  • Organization: BioTime



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Easy and quick RNA prep.
Rating: 4.0

  • Application Area:Gene expression profile of mammalian cells

"The kit was easy to use, and the results were comparable to previous RNA isolation, with the advantage of not needing to separate Trizol reagent first."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: BCM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The kit is great, inexpensive, and customer support was great.
Rating: 5.0

  • Application Area:Purify RNA from MACS sorted cells

"I have prepared up to 27 RNA samples at a time with this product and have so far had good reliable repeatable results. Couldn't ask for more."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Harvard



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great product.
Rating: 4.3

  • Application Area:RNA isolation from tissue samples

"Works very well with different tissues samples, very simple protocol, works with trizol lysates which avoids the need to use chloroform extraction, enrichment of small RNA."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Otago Christchurch



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great kit, will be using them for all RNA extraction.
Rating: 5.0

  • Application Area:RNA extraction. Needed pure RNA for nansotring

"Compared to traditional Trizol extraction, Direct-zol kits were easier and took less time. This helped me to extract RNA from more samples in less time. Extracted RNA was of better quality and saved me from repeating cell culture experiments, saving time and money. I will be using these for all my subsequent RNA extractions. I had great response from Sai Palagummi, prompt reply to my emails and offered good pricing and timely delivery. He also met to discuss my other experimental needs and gave me other products which are really crucial to my experiments. I am very happy with after-sales experience. "

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Bucknell University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Low cost RNA isolation.
Rating: 4.7

  • Application Area:RNA purification

"The protocol is a bit less user friendly than some of the other RNA isolation kits. But the kit does an excellent job of isolating high quality RNA that has excellent OD260/230 and 260/280 ratios. The included DNase works well most of the time, but sometimes an additional DNase step is required for some samples."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The George Washington University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Clean and fast result for your money.
Rating: 5.0

  • Application Area:Brain, fat and liver tissues

"I achieved much higher quality and for less time than with the other kits, or with conventional methods. Great value for money. I have recommended to other labs around as well."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Nevada, Reno



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Best RNA extraction kit I have used.
Rating: 5.0

  • Application Area:Analyze gene expression changes in Mouse hypothalamic tissue

"This is a great product for extracting RNA from tissue. We get a great yield and a very clean prep. Best kit I have used so far."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Atlantic Cancer Research Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results. Won't change company.
Rating: 5.0

  • Application Area:RNA extraction for next-generation sequencing and RT-qPCR

"Fast and easy to use. RNA output clean and good range."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NATIONAL INSTITUTE OF BIOLOGY



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
high RNA yields and quality, easy to use.
Rating: 4.3

  • Application Area:Isolation of totRNA from animal tissue

"It isn't cheap but you get good yields and high quality RNA without having to struggle with phase separation of trizol reagent. The instructions are clear and the reproducibility was good in my case."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Lund university



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
I am not changing from my old kit.
Rating: 3.7

  • Application Area:Gene expression in solid tumors

"Easy and fast kit, but not better than for example Qiagen RNeasy. Quality was not as good as after extracting with Qiagen."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Walter and Eliza Hall Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good value for money!
Rating: 5.0

  • Application Area:RNA extraction from samples with little input material

"I had little very starting material, and got a higher yield in RNA as compared to comparable kits from other brands. Quality of RNA was very good."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Dana-Farber Cancer Institute



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
High yield and great results.
Rating: 4.7

  • Application Area:RNA isolation from cells

"It is really simple to use and very efficient. The RNA quality was excellent."

Review date: 29 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Arcturus Therapeutics



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Great yield of large RNAs.
Rating: 3.3

  • Application Area:Gene expression and miRNA isolation

"It is easy to use and instruction is very clear. However, we experienced lower yield of short RNA than miRNeasy."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Virginia Tech Biology Department



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    4 out of 5
Great results.
Rating: 3.7

  • Application Area:Analysis of RNA quantity and quality from avian tissue

"In direct comparison to the equivalent QIAGEN product, this product gave excellent results. Simple protocol makes it easy to use."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Maine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
So happy with kit, and the customer service is awesome!
Rating: 5.0

  • Application Area:Extract RNA from Zebrafish larvae for cDNA synthesis and qPCR

"The Direct-zol™ RNA MiniPrep is so much easier and faster to use than our previous RNA extraction kit. We are able to process more samples in a shorter amount of time, and we even receive greater yields (although they are about the same in terms of cleanliness of the sample). When I was having trouble extracting RNA from single larval Zebrafish samples, my customer service rep Danice was super helpful and even sent us some free beads and finer spin columns to try. Although we didn't get the results we hoped for, it was still awesome that the company was willing to work with us to solve our problem. I would definitely continue to use this product in my research going forward and would recommend to others."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results
Rating: 5.0

  • Application Area:RNA extraction

"This kit is very convenient. I save a lot of time."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Cape Town



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Most reliable with good quality results, easy to use and cost effective.
Rating: 4.0

  • Application Area:RNA extraction and purification

"Very effective with high quality extraction efficiency. It’s very quick and reliable and cost effective. I highly recommend it for researches especially for plants and fungal RNA extraction."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of California, San Diego



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Life-changing! Life before Direct-zol wasn't worth living!
Rating: 4.7

  • Application Area:Extract RNA from tissues

"Direct-zol™ RNA MiniPrep has been a life-changing product for me. Gone are the days of having to just hope when extracting a small amount of RNA using phenol-chloroform. The product is reliable and easy to use; this has allowed me to trust junior students with RNA extractions with confidence."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Texas A&M



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results.
Rating: 5.0

  • Application Area:Lyme disease

"The purity of total RNA was perfect. I had to use it for RNA sequencing and have no complains."

Review date: 28 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Nebraska Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good quality RNA, and makes life easier.
Rating: 5.0

  • Application Area:RNA isolation

"The product allows for quick RNA isolation without sacrificing quality. In column DNase treatment also speeds up the process from purification to making cDNA. It works well when isolating RNA from limited number of cells such as 6,12,24 well dishes."

Review date: 27 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Rochester Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Quick with great results!!!
Rating: 5.0

  • Application Area:RNA Extraction

"The product was very easy, user friendly and QUICK!! I was skeptical at first because most kits I've used takes about an hour. My entire lab uses just this now."

Review date: 27 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of California San Diego (UCSD)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product, RNA super clean in 10minutes! Cheap too!!
Rating: 5.0

  • Application Area:RNA extraction.

"This product is very useful since it gives 100% of the time clean RNA, no phenol contamination at all. Even though it uses columns I did not see any loss in quantity of RNA extracted. The thing I like the most is that it gets clean RNA. There is nothing that I don't like about this product. It is very reliable. It is super fast - in 10minutes you get your RNA. It is totally worth the price that is cheap. I would definitely buy it again and recommend to anyone (I already did). Very easy to use too."

Review date: 26 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Indiana University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    1 out of 5
Lousy performance in miRNA isolation.
Rating: 3.0

  • Application Area:Blood/serum miRNA.

"Awful/subpar performance in microRNA isolation from serum samples and exosome preps as compared to miRNeasy and assessed by qPCR, digital PCR and Bioanalyzer."

Review date: 26 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Arizona



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
A great step forward in RNA preps.
Rating: 4.7

  • Application Area:RNA from whole blood.

"Love this product. It's as easy to use as any other column type prep and turn out great yields and high quality RNA."

Review date: 26 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Michigan, Ann Arbor



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results, highly recommended kit
Rating: 4.7

  • Application Area:Analyze gene transcription in bacteria

"This kit significantly helps in generating RNA from bacteria that is both high quantity and high quality. The ability to extract RNA directly from samples stored in TRIzol using a spin column reduces both the time and potential for user error involved in what is generally a tedious process (phenol/chloroform extraction) that is prone to poor yields. Highly recommend also acquiring the kit that enables DNase treatment as part of the protocol -- it's not always 100% effective at removing gDNA contamination, but usually is sufficient. Overall it’s the only method I use now for RNA work."

Review date: 25 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: West Virginia State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results. Completely satisfied. No need to search for alternatives.
Rating: 5.0

  • Application Area:Gene expression

"This protocol I feel is easy to use, quick, great results so far for plant tissues. It can give output with all types of RNAs without genomic DNA contamination."

Review date: 25 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Iowa



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, easy and fast.
Rating: 5.0

  • Application Area:Analyze transcription in cardiac tissue.

"This product has easy to follow steps that are well detailed in the manual. It makes RNA isolation a breeze compared to standard TRIzol methods. This kit is convenient. The yield and quality is excellent."

Review date: 22 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: OHSU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Value for money, works well
Rating: 5.0

  • Application Area:Analyze differentiation markers

"I got a good concentration of RNA out of my tiny samples. And it is much cheaper than other brands"

Review date: 21 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: FSU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product
Rating: 5.0

  • Application Area:RNA for gene expression analysis

"Processed several samples and isolated high quality RNA in every case"

Review date: 21 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Institute of Cellular Biology and Pathology N. Simionescu



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good results
Rating: 5.0

  • Application Area:Analyze miRNA in serum samples

"Easy to use. Good results. Doesn't work without phase separation, though. I think the membrane of the column clogs with too much protein."

Review date: 21 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Texas MD Anderson Cancer Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great results - always the number one kit for RNA isolation.
Rating: 4.3

  • Application Area:Molecular biology.

"It is very efficient for total RNA isolation (including the small RNA species) from different tissue sources. With in-column DNase treatment, I never got any genomic DNA contamination. Also, it is cost effective. I always use this kit for my studies."

Review date: 20 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Chciago



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great product, easy to use. Not going back to old Trizol RNA protocol.
Rating: 4.3

  • Application Area:Immunology.

"I discovered this product during a vendor show. We give it a try and we're not going back to old Trizol RNA isolation protocol. It's easy to use and save so much time. The quality of RNA you get is always great, thus results are more consistent. I shared this to everyone in the lab and they all love it."

Review date: 20 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Oklahoma Health Sciences Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, easy to use. I highly recommend this kit!
Rating: 5.0

  • Application Area:RNA prep for downstream NGS library preparation.

"The Direct-zol™ RNA preps have been an excellent addition to our workflow for NGS library construction. The protocol is simple and works on a variety of sample types (mouse brain, mouse intestine, cell prep, tissue, etc...). The kit always gives high yield, high quality material that works well in our downstream applications. I now recommend this kit to all of our core users."

Review date: 20 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The Ohio State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It's the easiest way to get great RNA. Each column has a max of ~50 μg.
Rating: 5.0

  • Application Area:Purify high quality and purity RNA from cells and cell lysates.

"Since using this product, I never do traditional trizol anymore, only RNA purification. EVER. Reproducible clean results with basically ZERO protein or organic contamination, and no need to sacrifice a portion of your sample to phase separation."

Review date: 18 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Instituto de Medicina Molecular



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent results, good quantity and quality of the obtained RNA.
Rating: 5.0

  • Application Area:To extract RNA from tissue and cell samples, for qRT-PCR.

"The kit is very easy to use, with a simple protocol. It has the big advantage of being used both with tissues and cells, something that doesn't happen with kits from other brands. The possibility of being used with TRizol or equivalent reagents is also a plus, since TRizol allows us to obtain higher quantities compared to the lysis buffers from other kits. The yield obtained in the end is bigger than with kits from other brands that we tried before. The price of the kit is good when we consider the price/quality ratio, in comparison to other options in the market. In regards to the after sales care, the attention to the client was always good and I never had any problems when asking for support."

Review date: 14 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: univ chicago



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great stuff. Will use again.
Rating: 5.0

  • Application Area:RNA isolation.

"I bought this kit for RNA isolation on an array and it worked great. Quality and quantity were both reproducible and RIN was 8 and above."

Review date: 14 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CINCINNATI CHILDRENS HOSP MED CTR



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Good results.
Rating: 5.0

  • Application Area:RNA isolation for gene expression study.

"The product is good value for the money, but the yield is comparably lower with other similar products. I used this kit for regular RNA isolation. You would get enough RNA for cDNA synthesis, but it would be better if the yield is higher."

Review date: 14 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Alberta



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent product; easy to use and produces high quality RNA.
Rating: 5.0

  • Application Area:RNA-seq for Differential Gene expression analysis

"Direct-zol was a very easy to use product that produced consistent, high yield and high quality RNA. These results were confirmed using Nanodrop and an Agilent 2100 Bioanalyzer. This product is comparable in cost, but saves a large amount of time in process, making it well worthwhile. I will continue to use Direct-zol in future experimentation. "

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NYU Medical Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Excellent kit for quick and easy isolation of high quality RNA.
Rating: 5.0

  • Application Area:Isolation of RNA from cancer cell lines.

"An excellent kit for preparing high quality RNA for RT-PCR or RNAseq applications. Very clear protocol, RNA isolation is quick and straightforward. Definitely recommend."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The University of Georgia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Quick and Easy to Use Kit With Quality Results
Rating: 5.0

  • Application Area:Analyze gene expression in tissue samples

"I was amazed at how efficient and easy this kit was to use. I work with plant tissue and bacterial cell cultures and I can achieve high quality and quantity RNA in under 40 minutes. My results are reproducible and reliable. I would highly recommend this kit to any laboratory looking for a good kit to isolate RNA quickly."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of California, Berkeley



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Fundamental for my research
Rating: 4.7

  • Application Area:transcriptional profiling of bacteria-host interactions

"This product has the best yield and reproducibility I've seen in many related kits, when I had some problems with a batch, the customer care promptly responded to my questions and provide me with alternatives."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Université François Rabelais



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very mRNA quality and quantity.
Rating: 4.3

  • Application Area:Lipidomics in cancer

"This easy-to-use kit allowed me to obtain high quality mRNA with reproducible results. Thus, the number of assays could be reduced."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NJH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Makes my life easier...best for Human sputa in trizol
Rating: 5.0

  • Application Area:Trizol prep of Human Sputa

"Best product ever for my needs. Makes processing human sputa in TB research very easy"

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Southern Utah U



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Perfect kit.
Rating: 4.7

  • Application Area:Transcript analysis and rt-pcr

"Easy to use. High yield. Relatively inexpensive. And reproducible results."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cancer Research Center, Campus Miguel de Unamuno, 37007 Salamanca, Spain



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Product is great, very easy and quick to get very high quality RNA preserving miRNAs
Rating: 5.0

  • Application Area:Analyze miRNA expression in primary cells and tumor cell lines

"You can very easily and quickly obtain high quality RNA (assessed by Agilent) from cells directly from TRIzol or similar reagent. The best is that both mRNA and miRNAs are preserved."

Review date: 13 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Oakland University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great product!!
Rating: 4.7

  • Application Area:RNA purification

"It is very easy to use, and the whole process take a few minutes, with great RNA quality at the end"

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Zaragoza, Spain



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Mostly great and consistent, new DNase treatment improved
Rating: 4.3

  • Application Area:Microrna research

"Easy and fast, new DNase treatment is an improvement. I find this one of the best kits for RNA/miRNA preparation from solid tissues including muscle and CNS."

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: FIU



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Extremely effective and easy to use kit.
Rating: 4.7

  • Application Area:Transcriptome studies using RNA-seq, Microarray

"In the last few years we have been using the Zymo Research DirectZol RNA minikit for our RNA work. In my experience, as of now this kit has given us some best quality RNA as compared to the others. The RNA can be processed or directly used for a number of downstream stream applications including, but not restricted to, qPCR and high-throughput RNA-seq transcriptome studies. The DirectZol-RNA minikit can be used to extract RNA from a wide range of samples and is very easy to use. Good quality of RNA can be extracted in a very less time using this kit. Overall, it is a very effective and efficient kit. "

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Washington



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Another good kit for RNA isolation
Rating: 4.3

  • Application Area:RNA extraction from mouse tissue

"Easy process. Yield comparable to other kits, but not better."

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Montreal



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great product
Rating: 4.7

  • Application Area:RNA isolation for RT-PCR

"Very effective, high yield and reproducible results "

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: St. Jude



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
It is pretty simple/easy but high quality performance kit...
Rating: 5.0

  • Application Area:RNA expression level with qPCR

"I have been used this kit last 5 years...It is pretty easy to follow protocol but never failed your RNA preparation. I highly recommend.... "

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Chalmers University of Technology



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Extremely fast, good for most applications
Rating: 4.3

  • Application Area:RNA extraction; RN A-Seq

"We use it for most of our RNA-based procedures. It is extremely fast. Now we do not spend hours with the extraction"

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UNIVERSITY OF LEEDS



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
Good RNA quality!
Rating: 4.3

  • Application Area:analyze mRNA levels C. elegans stress

"Direct-zol RNA MiniPrep kit is one of the most efficient and time saving kits I have used to isolate total RNA from C. elegans."

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University Of Puerto Rico



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great result and very fast
Rating: 5.0

  • Application Area:RNA extraction

"The results are very High quality. Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol, the experiment has a phase separation and precipitation procedures, for non-biased recovery of miRNA. "

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Wisconsin Madison



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great for price but not always compatible with qiacube
Rating: 4.0

  • Application Area:RNA extraction

"Very similar levels of RNA as compared to QIAGEN Rneasy mini. Used to work with QIAGEN’s qiacube but inconsistent. "

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very effective and easy to use product
Rating: 5.0

  • Application Area:RNA extraction from samples in TriZol

"Very cost effective and easy to use product. Convenient on column DNAse incubation option and particularly good to retrieve small or fragmented RNA."

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Connecticut



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great results, easy to use!
Rating: 5.0

  • Application Area:RNA extraction

"This product was exceptionally easy to use and provided us to ample high quality RNA every time. This RNA was then used for illumina RNAseq. Would highly recommend this to anyone performing RNA extractions."

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UTSW



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Never want to use any other kits
Rating: 4.7

  • Application Area:RNA from intestines

"Great product. Wonderful quality and quantity. Extremely easy and very quick. Love it!"

Review date: 12 Jul 2016 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: University of Georgia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:RAN isolation

"This mini prep kit is extremely easy to use and time saving, compared to other alternatives like making your own isolation recipe - which might be cheaper - but time consuming. I have also had very good results isolating different RNA sizes compared to my conventional method. Note: I didn't contact after sales but I voted them 5 stars since there was no "inapplicable" option. "

Review date: 18 Feb 2015 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Neuroscience

"This kit is easy to use and has all the advantages of phenol: chloroform extraction with much less hassle. The RNA extraction with this kit takes less than 5 minutes, either directly from the Direct-zol(TM) itself or from the chloroform step. If you take the RNA from the chloroform extract, then you can still extract the protein and DNA. Furthermore, the RNA is high quality and gives good spectrophotometer readings (the 260/280 and 260/230 readings which are typically close to 2.0). This kit is cheap and works well. I highly recommend this for anyone who is looking to extract RNA and continue to use their phenol: chloroform extraction reagents."

Review date: 20 Dec 2013 | Direct-zol™ RNA MiniPrep
  • Status:

    Reviewer

  • Member since: 2003

  • Organization: Biozenrum



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Molecular Biology

"My experience with this kit is very positive. The trizol lysate is directly loaded onto the column without prior phase separation. Also, the small RNA are preserved (co-isolated) with total RNA even when DNAse I is performed. However, the only drawback is you need to make sure you don't over saturate the column."

Review date: 14 Jan 2013 | Direct-zol™ RNA MiniPrep

 

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar.

 

Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary.

Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

 

Features:

  • The entire procedure typically takes about 5-10 minutes
  • Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar
  • Bypasses phase separation and precipitation procedures
  • DNase I included for DNA-free RNA

 

Product Overview

Product Image

Direct-zol™ RNA MiniPrep

Manufacturer Zymo Research

4.7 / 5.0 | 66 reviews