DAWN™

Characterizing complex biologics demands precision, traceability, and streamlined workflows across development and QC. Integrating SEC-MALS with Empower™ Software addresses these challenges by uniting robust data acquisition with advanced light scattering analysis.

Discover how this technology enables seamless method transfer and reliable characterization of protein standards, enhancing regulatory compliance and analytical confidence in biopharmaceutical workflows.

In biotherapeutics manufacturing, controlling the size distribution of therapeutic monoclonal antibodies (mAbs) is critical to ensuring product quality. Size-exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) is a leading method for analyzing size variants by separating high and low molecular weight species from the monomer based on their hydrodynamic volume.

Learn how the analytical team at Tanvex BioPharma, Inc. found SEC-MALS to be an advantageous method which is cost-effective and efficient for analyzing size variants across a broad range of mAbs.

Reliable biophysical characterization is a cornerstone of biologics development, playing a critical role at every stage – from selecting promising drug candidates to optimizing formulations and ensuring consistent manufacturing processes. Explore a comprehensive guide on how to deliver biopharmaceutical solutions, beginning with light scattering technology.

Explore the impact of gamma irradiation on sodium alginate, a polysaccharide widely used in food and medical applications. Using SEC-MALS with a DAWN™ multi-angle light scattering detector and Optilab™ refractometer, researchers can determine molecular weight, size, and conformation without relying on column calibration assumptions. Understanding these properties is crucial for optimizing sodium alginate's performance as a thickening agent and sterile wound dressing.

Chondroitin sulphate is started being used to treated people suffering arthritis, however Different preparations of the product have different molecular properties which are related to therapeutic effectiveness. In this application note, different chondroitin sulphate samples were compared using using size-exclusion chromatography (SEC) coupled to a DAWN multi-angle light scattering (MALS) and a WyattQELS quasi-elastic light scattering detector (QELS). The SEC-MALS-QELS method provides complete information about molar mass, radius and distributions of chondroitin sulfate.

This application note describes the use of multi-angle light scattering (MALS) for characterizing the intermolecular interactions between three different high concentration antibody solutions using a Calypso II, a HELEOS II MALS detector, and Optilab T-rEX™ dRI detector. CG-MALS measurements, automated by Wyatt equipment, provided insight into the colloidal behaviors of the antibody solutions and the nature of the intermolecular forces that contribute to high viscosity.

The biopolymer hyaluronic acid is present in all mammalian systems and is of enormous commercial interest for opthalmic, medical, pharmacological and cosmetic applications. In this application note, the absolute determination of hyaluronic acids' properties is demonstrated using MALS, differential refractometry, and differential viscometry detectors in series with SEC separation.

In this technical note, Wyatt Technology lists patents and patent applications using CG-MALS. Where patent applications have been submitted to multiple countries or organizations, only one is mentioned.

In this technical note, Wyatt Technology lists key publications using CG-MALS for the characterization of specific heteroassociation in proteins and other biomacromolecules.

In this technical note, Wyatt Technology lists key publications using CG-MALS for the characterization of specific self-association in proteins and other biomacromolecules.

Tuesday, February 10 at 16:00 GMT | 17:00 CET | 11:00 EST | 8:00 PST

High-concentration antibody formulations provide patients with the flexibility of self-administered subcutaneous dosing. However, achieving stability and manufacturability of these solutions can lead to increasingly complex formulation development. With expansive libraries of protein variants and formulation conditions to evaluate, researchers need smarter, faster, and more comprehensive analytical tools to ensure chemical stability and optimal delivery characteristics.

Join Dr. Sophia Kenrick of Waters | Wyatt Technology and Dr. Christine Nervig of TA Instruments as they present complementary approaches to stability characterization, leveraging high-throughput technologies:

1. High-throughput dynamic and static light scattering (DLS/SLS): These techniques provide rapid, sensitive indicators of aggregation and colloidal stability across a wide range of conditions.

2. Rapid screening-differential scanning calorimetry (RS-DSC): This next-generation DSC platform enables simultaneous, low-volume measurements to assess protein unfolding and conformational stability with exceptional throughput.

Discover how these complementary methods offer a high-resolution, high-throughput solution for evaluating  thermal, colloidal, and long-term stability, to empower scientists to make faster, data-driven decisions in the development of stable, effective biologics.

Key learning objectives:

• Understand how protein stability influences candidate and formulation selection during biotherapeutic development.
• Recognize the benefits of high-throughput analysis for accelerating workflows in the biopharmaceutical industry.
• Learn how DLS/SLS and DSC can be applied to comprehensively assess thermal, colloidal, and long-term stability.

Who should attend?

• Formulation scientists and protein engineers involved in developing high-concentration biologics.
• Analytical and biophysical characterization teams responsible for evaluating aggregation, conformational, and conformation stability.
• Biopharmaceutical R&D leaders and project managers overseeing biologics development pipeline looking for orthogonal approaches that streamline collection and decision making.

Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Heightened interest in mRNA therapeutics has accelerated the need for analytical methodologies that facilitate the production of supplies for clinical trials. This webinar will present a concise panel of chromatography-based stability-indicating assays for evaluating thermally stressed in vitro transcribed mRNA as part of a forced degradation study.

Our expert speakers will demonstrate the application of these methods to evaluate the critical quality attributes (CQAs) of mRNAs as well as to detect intrinsic process- and product-related impurities.

Key learning objectives

• Characterization of thermally stressed mRNA using a panel of chromatographic assays
• Implementation of SEC-MALS to confirm transcripts reflect full-length sequence design
• Platform 5’ cap/3’ tail analyses, circumventing antisense probe design
• Design of QC-friendly methods fit for research, release, and comparability testing

Who should attend?

• QC lab technicians or lab assistants.
• Lab managers of development, process development, or QC.
• Directors/Senior directors of development, process development or QC
• VP of Quality; VP of Development or VP of CMC.
• Anyone interested in learning more about light scattering and its applications on mRNA characterization.
• Industry and academic researchers working in gene therapy and biotherapeutics.
• Any scientist in discovery, development, and quality control.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

March 19, 15:00 GMT/ 16:00 CET/ 11:00 EDT/ 08:00 PDT

AAV-based gene therapy products are heterogeneous, composed of empty, partial, and full monomer capsid species as well as capsid aggregates and fragments. Join our exclusive webinar where Dr Margaret Butko, Director of CMC Assay Development, Adverum and Dr. Prithwijit Sarkar, Sr. System Account Manager, Waters|Wyatt Technology, will present two methods: GMP-compliant DLS (dynamic light scattering) which is used for monitoring capsid aggregation, including generation of a capsid aggregation standard, and the FFF-MALS (field-flow fractionation coupled with multi-angle light scattering) method, used for quantifying the relative abundance of capsid aggregates.

Explore these two methods, with the addition of quantification platform SEC-MALS (size exclusion chromatography coupled with multi-angle light scattering), to quantify the relative abundance of both capsid aggregates and full capsids. Find out how you can elevate your AAV-based gene therapy research with these tools and ensure the delivery of consistent products to patients.

Key learning objectives:

• Explore DLS applications to monitor capsid aggregation in a GMP setting
• Discover FFF-MALS applications to quantify the relative abundance of capsid aggregates
• Investigate SEC-MALS applications to quantify the relative abundance of full capsids

Who should attend?

• CMC teams supporting AAV-based therapies
• Scientists involved in AAV characterization, analytical method development, process development, and quality control
• Lab managers that need to select and support an optimal set of AAV characterization methods

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

As a core analytical facility, we are approached by users seeking our services with a large variety of samples. They usually have specific experiments/equipment in mind, frequently desiring to use the most intensive experimental techniques (in terms of sample, time, and money), when a simpler technique could give them the answers they require at a fraction of the cost. Often our recommended techniques include preliminary analysis with dynamic light scattering (DLS), followed by multi-angle light scattering (MALS), instead of analytical ultracentrifugation (AUC) or other high-maintenance biophysical techniques.

In this webinar expert speakers will explain, with a few examples, the methodology we have developed to save users time, sample, and money by prioritizing certain techniques for preliminary experiments. Many times the results from these ‘preliminary’ experiments are sufficient to provide the answers to the questions our researchers are asking.

Key learning objectives

• The fundamentals of light scattering theory, instrumentation and applications
• How light scattering instruments are used for fast, preliminary experiments before more intensive studies are attempted
• How often preliminary experiments provide all the answers needed for the scientific question
• Advantages (and some disadvantages) of Wyatt’s DLS and MALS instruments in relation to other techniques such as AUC.

Who should attend?

• Customers of biophysical core facilities or CROs who are considering light scattering in relation to other techniques
• Lab managers responsible for biophysical characterization who would like to optimize facility workflows, productivity, and use

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

This year's winner of the Reviewers’ Choice Award™ for Instrument of the Year was Wyatt Technology Corp for its DAWN HELIOS II Multi-Angle Light Scattering Detector. Hear CEO Phillp J. Wyatt's reaction to winning this award for a product which is designed to provide high sensitivity and a wide range of measurements.

Explore innovations such as oligonucleotide therapeutics, bi-specific antibodies, CAR-T therapy and CRISPR

A panel of experts share their insights into the development of lipid nanoparticles for drug delivery and reveal the analytical solutions designed to help labs advance further in this field.

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