CyGEL
A novel thermoreversible gel mountant for temporary immobilization and imaging.
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Excellent product to safely immobilizes motile cells
Live cell fluorescent imaging of Leishmania promastigotes
Without Cygel it would be almost impossible to obtain high-quality microscopy of motile forms of Leishmania parasites. The product is easy to use and gives a reproducible performance. I've used it to image Leishmania promastigotes that were modified to contain fluorescent proteins, in order to gain information on protein function. Cellular morphology remained unchanged by the embedding process and protein localization was not affected. Can highly recommend Cygel.
Review Date: 19 Aug 2022 | Biostatus Limited
Excellent results.
Leishmania mexicana live cell imaging
CyGel was very convenient to use and I would recommend it to everyone working on leishmania.
Review Date: 25 Oct 2019 | Biostatus Limited
Excellent for mounting live cells for microscopy.
Mounting bacteria for live cell confocal microscopy
Cygel has been a great addition to my microscopy toolbox! It enables me to carry out imaging of motile bacteria without needing to fix them. Very easy to use, and seems to go a long way. Can highly recommend.
Review Date: 8 Jun 2019 | Biostatus Limited
Excellent product, affordable, easy to use, and gave great results.
Imaging non-adherent leukemia cell line for biosensor application.
We used CyGel for imaging a K562 cell line to verify that our fluorophore-tagged biosensor is internalized by the cells. The product was very easy to use and worked according to the manufacturer’s description. There was no problem with CyGel interfering with our fluorescent signal using a standard fluorescent microscope for cell imaging. We think it was a very reasonable price for the ease of use and results we obtained. While we did not require follow up service, it was there for our assistance as needed.
Review Date: 19 Sept 2016 | Biostatus Limited
I achieved reproducible results in imaging and rescuing zebrafish larva using CyGel.
Live Imaging of Zebrafish Larva
Imaging mitochondrial dynamics requires that my sample be completely stationary and alive! CyGel allowed me to stabilize zebrafish larva and obtain time-lapse images of mitochondria. I was able to rescue the fish afterwards, so that fish could be imaged on multiple days if necessary.
Review Date: 8 Aug 2016 | Biostatus Limited
Useful product
frog embryo research
This is an effective product that we find is relatively easy to use.
Review Date: 13 Jun 2016 | Biostatus Limited
Reliable, user-friendly product
Live cell imaging by confocal microscopy
We have used CyGEL to immobilize Drosophila pupae for live imaging using a confocal microscope. Embedding of samples in glass bottom dishes is carried out using a fluorescence stereomicroscope. We find CyGEL more convenient than LMP agarose because it saves the hassle to melt agarose and have a heating block or water bath nearby to keep agarose liquefied. The only utensil required is a small container with ice to keep CyGEL in its liquid form. Toxicity was not an issue since samples survived 3D time-lapse imaging for five days (see http://rsos.royalsocietypublishing.org/content/3/2/150517). Overall, we are very satisfied with this product.
Review Date: 26 Feb 2016 | Biostatus Limited
Confocal microscopy
This is wonderful product to immobilize cells. It can be used also for immobilization of cells after cell sorting. Just sort cells on cool microscope slides and your cells will be immobilized during transport to confocal microscopy (as the slides will heat to room temperature). Just a very easy-to-use tool.
Review Date: 9 Oct 2014 | Biostatus Limited
A thermoreversible gel compatible with LIVE cells.
It can be used to immobilise non-adherent cells and beads by simple warming, and conversely allowing their recovery by simple cooling.
CyGEL™ is unusual in that it is a liquid when cold and a gel when warmed!
What are the key benefits?
CyGEL™ has many applications in imaging, flow cytometry and cell-based screening. It is compatible with LIVE cells, usefully allowing their immobilisation for analysis and subsequent release for ongoing experimentation.
Key benefits include:-
- Optically clear with low autofluorescence
- Controllable and rapid transition from liquid to gel
- Convenient immobilisation of non-adherent cells and beads
- Compatible with GFP and "in gel" fluorescent probes including DRAQ5™
Advantages:
- Allows rapid preparation of cells for gel mounting
- Permits long term analysis of non-adherent cells
- Acts as a support matrix
- Permits sterile, low temperature recovery of cells from gel
- Multiple assay formats and applications including apoptosis detection
CyGEL Sustain™ in Image-Based Apoptosis Assays: Extended Immobilization of Non-Adherent / De-Adhering Cells
Microscopy offers the ability to gain important morphological information, including nuclear appearance to identify apoptotic events, or even subcellular location of other markers of interest. Cells undergoing death processes modify both localization and mobility properties of DNA-binding proteins and other compartmentalized and membrane‐located proteins allowing stages of apoptosis to be resolved. Sometimes there is a need to immobilize cells or even whole organisms over several hours to analyze events or signals of interest. This application note demonstrates how CyGEL Sustain™ provides a long-acting thermoreversible hydrogel mountant for non-adherent/motile cells.
CyGEL<sup>TM</sup> in Immunofluorescence: Mounting Non-Adherent Cells / Fragile Objects
Immunofluorescence microscopy allows the detailed 2-D examination of adherent cell phenotypes and tissue sections that have been preserved by fixation with formaldehyde or similar. The problem arises when non-adherent cells are to be analysed by fluorescence microscopy. Also, in the case of fragile tissue pieces these can be damaged by efforts to make them mount flat after already processing them for immunofluorescence labeling. An alternative provided by CyGELTM is to capture all cells after staining in an immobilizing, optically compatible hydrogel which can then be deposited into a slide or chambered coverslip. The use of CyGELTM in mounting adherent cells/fragile objects for imaging is described in this application note.
Image Immobilised Cells with New Microtitre CyGEL™ Technology
Learn about the new microtitre format for the CyGEL™ hydrogel technology, enabling you to look at the cell biology of true interest. SelectScience.tv interview with Biostatus at SBS Annual Conference 09.
