CyBio Well Vario

In this application note, the CyBi®-Well vario Nanoliter Head 384/2.5μL was used to determine the liquid handling precision with fluorescence readout in the low volume range, using buffer and DMSO in the wet-to-wet liquid transfer mode. The CyBi®-Well vario has six pipetting heads which allow for fast and precise simultaneous pipetting whilst providing reliable handling of different liquids over a broad volume range.

In this application note the tip washing efficiency of the active deep well Tip Wash Station 384 was determined quantitatively with optimized pump settings and different washing liquids after different numbers of washing cycles using a fluorescence read-out. Furthermore the start-up routing for the active deep well Tip Wash Station 384 was described in detail.

In this study the CyBi®-Well vario 96/25 µl pipetting head was used to determine the liquid handling precision with a fluorescence readout over a broad volume range using different pipetting modes, different disposable tips and liquids with different surface tensions. The data show the excellent pipetting performance of the CyBi®-Well vario 96/25 µl pipetting head under various conditions and provide a tool that supports the choice of the right experimental settings and disposable tips or a broad range of customer specific applications.

In the field of gene silencing, RNA molecules of 21-28 base pairs in length are used for the inhibition of gene expression in mammalian cells because long dsRNA molecules induce the mammalian interferon response. These molecules are termed small interfering RNA (siRNA). One effective method for delivery of siRNA into cells is transfection through lipofection. This method is believed to be especially effective in the cell growth phase. This is achieved by forming a transfection complex of siRNA and a cationic lipid-based transfection reagent which is then incubated with cells to deliver the siRNA molecule into the cytoplasm. This poster demonstrates the suitability of the CyBi®-Well Workstation for automated dispensing of samples for siRNA lipofection.

The accuracy and precision demands of liquid handling tasks are growing with increasing throughput and decreasing volumes. In this study the sophisticated and well accepted ARTEL Multichannel Verification System (MVS®) was used to evaluate the precision as well as the accuracy of the 384/25μl head of the CyBi®-Well vario, one of the most frequently used pipetting heads of CyBio´s flexible simultaneous pipetting platform.

This application note describes the HTS set-up of the ADME/Tox determination of human serum albumin binding using TRANSIL® HSA Binding Kit and the CyBi-Well® Vario with the 384/25 µL head.

Analytik Jena and Bruker have collaborated to bridge the gap between mass spectrometry’s powerful, label-free detection capabilities and modular automated uHTS sample preparation.

Join Dr. Christian Klinner, Analytik Jena, Arndt Asperger, Bruker Daltonics and Amanda Weaver, Biodesix, and as they discuss how the CyBio Well Vario pipetting device by Analytik Jena enhances MALDI spotting and sample optimization, seamlessly integrating with Bruker MS solutions to overcome application-specific bottlenecks.

Key learning objectives

• Learn how to increase your MALDI throughput, MALDI spotting of 1536 samples in <10 minutes
• Discover how to successfully overcome hardware, software and labware challenges
• Gain insights into a walk away automation solution for MALDI-TOF-MS

Who should attend?

Users looking to automate their MALDI-TOF sample prep

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

This video demonstrates how CyBio can help to maximize your productivity at every level of laboratory automation.

Watch this video to see how the new 1536-channel pipetting head uses CyBio's unique and well-proven tip sealing system. The 1536 air displacement pistons allow the simultaneous transfer and filling of all wells of a 1536-well plate in one single step.

Watch this video to learn more about the Plate Pooling customized compound management system from CyBio. This high-throughput system combines the automated reformatting of microplates with additional liquid handling steps, capping and decapping of source tubes, sealing of destination plates and barcode labelling.

Watch this SelectScience.tv interview at Drug Discovery 2009 (ELRIG) to learn how to enhance precision and reproducibility with CyBio’s Capillary Well Vario System. This system has a multi-channel capillary head and stackers to increase throughput and provide automated washing or barcode reading. Heads are also changeable from capillary to microlitre, allowing transfers of larger volumes.

Demonstration of the high performance and flexible pipetting platform of the CyBi®-Well, with a series of exchangeable heads. Heads are available in 96 and 384 tip configurations for a variety of volume ranges and different pipetting methods.