Pkg of 1, 7.5% precast polyacrylamide gel, 12+2–well, 45 μl, for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers. The new Criterion TGX (Tris-Glycin...read more
Pkg of 1, 7.5% precast polyacrylamide gel, 12+2–well, 45 μl, for use with Criterion and Criterion™ Dodeca™ electrophoresis cells and stain-free enabled imagers.
The new Criterion TGX (Tris-Glycine eXtended) Stain-Free precast gels for PAGE are based on the long shelf TGX formulation and include unique trihalo compounds that allow rapid fluorescent detection of protein with the Gel Doc EZ and ChemiDoc™ MP imaging systems.
Fast run times with protein separation, gel imaging, and complete analysis in less than 30 minutes
Rapid fluorescent detection with the Gel Doc EZ and ChemiDoc MP imaging systems
Comparable results to Coomassie stain
Better reproducibility and quantitation compared to other staining procedures
Same gel can be used for staining, western blotting, or mass spectrometry
TGX Stain-Free gels retain Laemmli-like separation characteristics using standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 minutes using the Criterion electrophoresis cell and then visualized with the Gel Doc EZ and ChemiDoc MP imagers in as little as 2.5 minutes.
The trihalo compounds react with tryptophan residues in a UV-induced reaction to produce fluorescence, which can be easily detected by the Gel Doc EZ and ChemiDoc MP imagers within gels or on a PVDF membrane after transfer.