7.5% Criterion™ TGX Stain-Free™ Protein Gel, 12+2 well, 45 µl
Pkg of 1, 7.5% precast polyacrylamide gel, 12+2-well, 45 µl, for use with Criterion electrophoresis cells and stain-free enabled imagers
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Use this midi Criterion TGX Protein Gel for separation of polypeptides >30 kD. The novel formulation of TGX, Tris-Glycine eXtended, Stain-Free Gels delivers a long shelf life and allows rapid fluorescent detection of proteins with Bio-Rad’s stain-free enabled imaging systems. TGX Stain-Free Gels can be used for either standard SDS denaturing protein separations or native electrophoresis.
Bio-Rad offers a wide range of Criterion TGX Stain-Free Precast Protein Gels, including other 12+2-well gels in both single percentages and gradients.
Run up to four gels using the Criterion Cells, or for higher throughput the Criterion Dodeca Cell can run up to 12 gels or IPG strips.
Features and Benefits
- Stain-free imaging of gels and blots in 1 min
- Can be used for denaturing electrophoresis with standard running buffer containing SDS
- Laemmli-like separation in the presence of SDS
- Run time as short as 20 min
- Proteins retain native conformation and activity in the absence SDS
- 12 month shelf life
Criterion TGX Stain-Free Gels contain trihalo compounds, which in the presence of UV-light react with tryptophan residues producing fluorescence. The fluorophores remain covalently bound to the proteins throughout electrophoresis and blotting, and may be visualized in gels or multiple times on PVDF membranes.
Related Products
- 10x Tris/Glycine/SDS Running Buffer (1610732)
- 10x Tris/Glycine Running Buffer (1610734)
- 2x Laemmli Sample Buffer (1610737)
- Native Sample Buffer (1610738)
- Protein Ladders and Standards (Markers) including unstained, prestained and western
- ChemiDoc™ Touch Imaging System and other ChemiDoc and GelDoc™ stain-free enabled imaging systems
Supporting Documents
Criterion Gel Application Guide, Instruction Manual
General V3 Western Blotting Protocol
Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls. Bio-Rad has developed the V3 Western Workflow™ utilizing stain-free technology to address the major concerns associated with the traditional western blotting procedure. This application note describes the V3 Western Workflow affording faster, more transparent, quantitative, and more reliable western blotting.
