CFX Connect™ Real–Time PCR Detection System (185-5200) by Bio-Rad

Manufacturer Bio-Rad
4.3
/
5.0
  |  7 reviews


CFX Connect™ Real–Time PCR Detection System (185-5200) by Bio-Rad product image
CFX Connect™ Real–Time PCR Detection System (185-5200)
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Average Rating: 4.3
7 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Simple to use and great results!
Rating: 4.7

  • Application Area: Gene expression analysis

"We bought this instrument to substitute an old Bio-Rad fluorescent system. The instrument and especially the software are very simple to use, very customizable. The course with the Bio-Rad technician was very useful to understand all the features of the CFX system. We use it to analyze gene expression using SYBR Green technology. The instrument is equipped with a LED lamp that doesn't need the same maintenance as the old fluorescent lamp."

Review date: 27 Mar 2017 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: University College of Medical Sciences (University of Delhi)



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Without this instrument we are not published any paper from our lab after its purchase.
Rating: 4.7

  • Application Area:Gene expression study in various disease to establish disease etiology

"Bio-Rad real-time PCR machine is awesome in performance and handling. Very user friendly software. I have been using it for 2 years and to date, I face neither any problem or any complaint by my colleagues. In our lab this is one of the most important instrument which on six days a week. "

Review date: 03 Dec 2015 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: BYU



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.3

  • Application Area:determine RNA expression in tissues and cells

"very useful, easy to use and easy to manage for different preparations and projects."

Review date: 10 Nov 2015 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Reliance Lifesciences Pvt Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Quantification of host cell DNA & Genotyping

"Very Robust and user-friendly system with options for multi tasking. After sales support is very good. The results are reproducible and consistent with very easy to interpret interface. It is a total value for money instrument."

Review date: 31 Mar 2015 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Advanced Reviewer

  • Member since: 2012

  • Organization: Fidia Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Rating: 4.7

  • Application Area:Molecular Biology

"This is an easy-to-use, straightforward PCR thermal cycler widely used among molecular biology laboratories throughout the world. Whether you are performing gene expression analyses or copy number variation assays, with the CFX96 you can produce results in less than 30 minutes thanks to the fast thermal cycling; minimize sample and reagent usage, obtaining optimal results with sample volumes as low as 10 microliters, and analyse your results when and where you want with the software which can send an email notification with attached results when a run is finished. I want to highly recommend this product as a powerful tool for your genomic research, providing the reliability you need to boost your discovery! "

Review date: 22 Apr 2014 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Korea University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Rating: 4.0

  • Application Area:Quantification of mRNA

"This machine is good enough for me to use it, and so strongly recommended to other groups."

Review date: 18 Mar 2014 | CFX Connect™ Real–Time PCR Detection System (185-5200)
  • Status:

    Reviewer

  • Member since: 2007

  • Organization: University Georgia



  • Ease of use
    3 out of 5
    After sales service
    2 out of 5
    Value for money
    3 out of 5
Rating: 2.7

  • Application Area:Real-time PCR, qPCR

"Overall the instrument is handy but it does not allow you to run all samples amplified with primers tagged with all types of fluorophores. For example I cannot detect signal from PCR with Cy5 tagged primers. I would recommend making sure it filters for your choice of fluorophores."

Review date: 31 Jan 2013 | CFX Connect™ Real–Time PCR Detection System (185-5200)

Manufacturer's Response

"The three channel CFX Connect optical hardware is specifically designed to detect up to two fluorophores per well within an excitation/emission spectral range between 450-580 nm. This spectral range was selected to offer an affordable instrument that supports the most commonly selected dyes for duplex experiments and single color experiments using intercalating dyes. The spectral range for Cy5 is located in the far red spectrum. For greater flexibility in fluorophore selection and the ability to accommodate Cy dyes, the CFX96 Touch system offers the ability to detect the full spectrum of fluorophores from 450-730 nm.
Warmest regards, Rachel Scott"
The CFX Connect real–time PCR detection system offers two–target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch™ real–time PCR detection system.

The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real–time PCR needs.


The CFX Connect real–time PCR detection system offers:

  • Easy startup — obtain great results right away with factory–calibrated dyes, quick setup, and intuitive software
  • Effortless optimization — save time and reduce costs by optimizing assays in a single run using the thermal gradient
  • Powerful data analysis tools — quickly and accurately validate and analyze data with the advanced analysis modules of CFX Manager™ software
  • Accelerated publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbasePLUS software