CellTiter-Glo® Luminescent Cell Viability Assay

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 4.6/5.0 (5 reviews)

Promega Corp.

The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of met...read more

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Reviews

LAURA SAUCEDO-CUEVAS


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Member since 2010

Organization:
SPANISH NATIONAL CANCER RESEARCH CENTER
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RATING: 3.6

  • 5 out of 5EASE OF USE
  • 3 out of 5AFTER SALES SERVICE
  • 3 out of 5VALUE FOR MONEY




Review date: 28 May 2012

Application Area: Cell Biology
CellTiter-Glo® Luminescent Cell Viability Assay

"Quick and easy to use. Low variation between experiments."

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JENNIFER ATKINSON


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Member since 2011

Organization:
ST. JUDE CHILDREN'S RES. HOSP.
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RATING: 4.6

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 4 out of 5VALUE FOR MONEY




Review date: 11 Jul 2011

CellTiter-Glo® Luminescent Cell Viability Assay

"Excellent, sensitive reagent. Luciferase based. Very high Z values in HTS. More expensive than other reagents but well worth it for ease of use and quality of data."

Taranjit Singh Gujral


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Member since 2007

Organization:
Queen's University
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 12 May 2007

CellTiter-Glo® Luminescent Cell Viability Assay

Mohammed Kashem


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Member since 2007

Organization:
Boehringer Ingelheim
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 04 May 2007

CellTiter-Glo® Luminescent Cell Viability Assay

"A great reagent for running kinase/ATPase assays."

Thomas Fletcher


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Member since 2006

Organization:
Southern Research
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RATING: 5.0

  • 5 out of 5EASE OF USE
  • 5 out of 5AFTER SALES SERVICE
  • 5 out of 5VALUE FOR MONEY




Review date: 08 Jan 2007

CellTiter-Glo® Luminescent Cell Viability Assay

Description

 

The CellTiter-Glo® Luminescent Cell Viability Assay(a,b) is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.

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