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"The antibody was used according to manufacture recommendations. Cells were blocked in PBS 1X/ 1% goat serum for 1h and the antibody was diluted was 1:10 and incubated for 10 min in the dark. Nsecondary antibody was Alexa 488 diluted at 1:1000 for 1h at RT in the dark. Cells were mounted and analyzed for protein expression"
Review date: 11 Nov 2015 |
CD133/1 (AC133) Antibody Clone AC133 recognizes the glycosylated epitope 1 of the human CD133 antigen (CD133/1). The CD133 antigen is expressed on stem cells with hematopoietic and nonhematopoietic differentiation potential. Four monoclonal antibodies recognizing the CD133 antigen are available: clone AC133 (mouse IgG1), 293C3 (mouse IgG2b), W6B3C1 (mouse IgG1) and AC141 (mouse IgG1). These antibodies recognize two different epitopes CD133/1 (clone AC133 and W6B3C1) and CD133/2 (clone 293C3 and clone AC141), respectively.