Caspase 8 Colorimetric Activity Assay Kit, IETD

Caspase 8 Colorimetric Activity Assay Kit, IETD
by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany


Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free p...read more

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Description

 

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. Chemicon's Caspase-8 Colorimetric Activity Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. The free pNA can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity.

Product Overview

Caspase 8 Colorimetric Activity Assay Kit, IETD by EMD Millipore, a division of Merck KGaA, Darmstadt, Germany
Caspase 8 Colorimetric Activity Assay Kit, IETD

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