The B Cell Isolation Kit is an indirect magnetic labeling system to isolate untouched resting B cells from single-cell suspensions of lymphoid tissues. Highly pure resting B cells are isolated by magnetic labeling and depletion of CD43-expressing B cells (activated B cells, plasma cells and CD5+ B-1a cells) and non-B cells. CD43+ B cells, T cells, NK cells, dendritic cells, macrophages, granulocytes, and erythroid cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD43 (Ly-48), CD4 (L3T4), and Ter-119, as well as Anti-Biotin MicroBeads. The B Cell Isolation Kit includes the Biotin-Antibody Cocktail for labeling of non-target cells and Anti-Biotin MicroBeads.
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