Automated Droplet Generator (1864101)
AutoDG™ Instrument for automated droplet generation of up to 96 samples, for use with the QX200™/QX100™ Droplet Digital™ PCR Systems
Automated Droplet Generator
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Great results and highly sensitive system for gene expression analysis.
Generate the various droplets for gene expression analysis
Excellent system if you are working in a field where gene quantification needs to be measured with high sensitivity in an accurate manner. Great machine, easy to handle and to maintain. I am grateful to have this system in our workplace for the great results.
Review Date: 11 Dec 2020 | Bio-Rad
Pretty good instrument for multiple applications, easy to use.
Testing a variety of samples for ddPCR analysis
The droplet generator helps precise analysis with clear droplet units. When it is working well, it can take a lower limit of DNA concentration for successful testing. It is easy to use since all of the steps of droplet generation are automated after you start your run. However, it occasionally reports error messages and takes multiple running cycles to recover from causing an error.
Review Date: 14 Nov 2019 | Bio-Rad
The AutoDG Instrument partitions each sample in a 96-well PCR plate into ~20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. Every droplet serves as an individual PCR reaction in Bio-Rad’s Droplet Digital (ddPCR™) PCR System. After partitioning, the plate is sealed with the PX1™ Plate Sealer, and PCR is run to end point in a thermal cycler. The droplets in each sample can then be analyzed using a QX100 or QX200 Droplet Reader.
The AutoDG Instrument eliminates user-to-user variability, allowing you to fully exploit the precision of ddPCR technology by providing more consistent droplet counts across users and plates. The AutoDG Instrument also minimizes hands-on time: it takes less than 5 minutes to load the instrument, and no supervision or user activity is required during droplet generation.
Features
- Guides setup and loading with large, color touch-screen interface and LED lighting
- Generates droplets for 96 ddPCR reactions in under 45 minutes
- One AutoDG Instrument can generate sufficient droplets to keep 4–5 droplet readers running continuously
- Provides operational flexibility:
- User-definable plate layout with increments of 8 wells
- HEPA-filtered enclosure reduces the possibility of contamination and allows the system to be placed in any lab environment
- Compatible with both QX100 and QX200 ddPCR workflows
Applications
- Cancer biomarker studies and copy number variation
- Pathogen detection
- Next generation sequencing
- Gene expression analysis
- Environmental monitoring
- Food testing
For extended product descriptions and detail specification information, please visit Bio-Rad.com.
Multiplex Screening of KRAS Mutations in Cell-Free DNA Colorectal Cancer Samples
This application note describes a ddPCR KRAS Screening Multiplex Kit for screening actionable KRAS mutations using DeopletDigital PCR (ddPCR). The ability to screen for multiple KRAS mutations down to 0.2% in a single well reduces the likelihood that mutations will be missed in poor-quality cell-free DNA anf formalin-fixed, paraffin-embedded (FFPE) samples with limited amounts of amplifiable DNA.
Meet the next evolution of ddPCR solutions
Monday, October 27, at 15:00 GMT | 16:00 CET | 11:00 EDT | 8:00 PDT
This webinar will explore the insights from the ddPCR World on Tour event in London and introduce the next evolution of Droplet Digital™ PCR (ddPCR™) technology. You’ll hear directly from fellow researchers and pioneers in the field as they share how ddPCR has advanced their studies, from detecting rare mutations to improving transplant monitoring. Plus, discover how Bio-Rad’s latest systems deliver exceptional sensitivity, simplicity, and performance, enabling absolute quantification, scalable workflows, and reliable results with minimal sample input.
Key learning objectives:
- Explore the latest ddPCR innovations and how they improve sensitivity, reproducibility, and ease of use.
- Hear about practical applications of ddPCR, from rare mutation detection to transplant monitoring.
- Gain real-world insights through user testimonials on how ddPCR has advanced their research.
The session will conclude with a Q&A, giving you the chance to ask our experts questions directly and gain practical insights to apply to your own research. Don’t miss this opportunity to see how ddPCR can transform your experiments.
Certificate of attendance
If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.
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