High Throughput (HT) cloning methods have evolved into thousands of experiments being performed routinely by research labs with the aim of improving their drug development process and gain insights into complex genetic interactions. A key technology for HT cloning systems has come from Invitrogen with its simple, yet flexible Gateway® technology.

HAMILTON Life Science Robotics has taken this technology one step further by creating a cloning system for the automation of Invitrogen’s Gateway® technology. The core of this cloning system is the MICROLAB STAR liquid handling workstation equipped with monitored air displacement pipetting technology. This eliminates use of tubing, pumps or system liquids, thus significantly reducing the risk of cross-contamination.

Plating of the transformed bacteria is done on the workstation and the 8-well SBS format plates containing the bacterial colonies are later picked using EasyPick (HAMILTON’s integrated colony picker).

Every operation is monitored and tracked by HAMILTON’s Platetrack software, enabling complex process handling and comprehensive audit trails. The system is operated using an easy-to-use graphical user interface.

The fully automated Cloning System for Gateway® technology has been designed to be compact to save precious lab space.

Gateway® and E-Gel® are registered trademarks of Invitrogen™ Corporation, Carlsbad, California/USA.