Amicon® Ultra-4 Centrifugal Filter Units

Manufacturer MilliporeSigma
4.3
/
5.0
  |  1 reviews


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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


Rating: 4.3

  • Application Area: Protein Purification And Concentration

"Amicon® Ultra-4 Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins below 3 kDa. This filter device can be used to concentrate a pure protein with molecular mass above 3 kDa. The recommended velocity to spin this device is 5000 RPM, however when the filter is used for long time it may be useful to decrease this speed to 3000 RPM as there is always chance of loss of proteins after using it for a long time. The second more important thing is to fill this device to a maximum of 3.5 ml as there is a chance of filter loss above this volume.This is suitable for small scale protein concentration. I recommend it to use for protein separation and concentration and to store this device either in 20% Ethanol or 0.02% Na-azide solution."

Review date: 29 Jan 2014 | Amicon® Ultra-4 Centrifugal Filter Units
• High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
• High retentate recovery of >90%
• Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
• Heat-sealed membrane minimizes downstream extractables
• 100% integrity tested for reliable performance
• Convenient sample monitoring with translucent housing and volume gradations
• Direct pipette sample access eliminates processing step to recover concentrate
• High concentration factors of 80–100X