Bioneer’s AccuPrep® Genomic DNA Extraction Kit allows the extraction of total genomic DNA from whole blood, lymphocytes, mammalian tissue and cultured cells. Average yield of 6 μg of total genomic DNA from 200 μl of whole human blood and up to 20 μg from 5 x 106 lymphocytes, 25 - 50 mg mammalian tissue, or 104 -108 cultured cells.
AccuPrep Genomic DNA Extraction Kit employs glass fibers, fixed in the AccuPrep column, that specifically bind DNA in the presence of a chaotropic salt. Proteins and other contaminants are eliminated through a series of short wash-and-spin steps. Finally, the genomic DNA is eluted by a low salt solution, and is ready for immediate use. This process does not require phenol/chloroform extraction, alcohol precipitation, or other burdensome steps. This kit is suitable to use with whole blood treated with either citrate or EDTA.