Products & ReviewSeparations

Agilent 1290 Infinity III 2D-LC System

Agilent TechnologiesAvailable: Worldwide

Increase resolution where you need it with the 1290 Infinity III 2D-LC System, which offers comprehensive two-dimensional liquid chromatography (2D-LC), (multiple) heart-cutting 2D-LC analysis, and high-resolution sampling. This system allows you to use 2D-HPLC for making buffer separations compatible to MS detection, while easy-to-use software enables fast method setup and refined data analysis.

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Average Rating 4.7

|2Scientists have reviewed this product

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Analyze samples qualitatively/quantitatively with great reproducibility of the results.

 

Average Rating 5.0

Application Area:

Comparative study of analytical quality of biosimilars

This instrument helps in solving complex separations as well as their analysis. Also, it is useful in the separation of co-eluting compounds and achieves the highest peak capacity. The resolution/separation of biomolecules can be increased using heart-cutting and high-resolution sampling or comprehensive methods. The user-friendly software for fast method setup and refined data analysis.

Review Date: 23 Feb 2021 | Agilent Technologies

Average Rating 4.3

Application Area:

Biosimilars

This product is very useful for extensive characterization studies and It is more user friendly than other vendors. 2D-LC used for heart-cut comprehensive heart cut of single and multiple peaks characterization by various formats of chromatography like, Protein-A to IEX, Protein-A to RP-HPLC, IEx to RP-HPLC. Agilent should focus more on applications of Biosimilars and Agilent should provide more training on the instrument.

Review Date: 15 Jul 2015 | Agilent Technologies

For applications in biopharma, pharma, chemical and other industries with demanding conditions with respect to solvents, samples and reagents, use the 1290 Infinity III Bio 2D-LC System with biocompatible or bio-inert LC modules and biocompatible 2D-LC valves and sample loops.

Use multiple heart-cutting 2D-LC to solve complex separations, and comprehensive 2D-LC to analyze complex samples with many compounds. This technology offers the proven robustness of Agilent 1290 Infinity III systems, and with the Easy Start Service it is easier than ever to get the full 2D experience.

Features

  • From separation of a few co-eluting compounds to mixtures of highest complexity - Agilent 2D-LC solutions allow you to do 2D-LC your way, choosing from 2D-LC modes (multiple) heart-cutting with high-resolution sampling and comprehensive 2D-LC
  • Set up measurements easily with powerful Agilent 2D-LC software. Start with your 1D measurement, choose spots where you want to increase resolution, draw your second dimension gradient, and go
  • Get all the information you need from your sample from qualitative results, including spectral data up to reproducible quantitative results
  • Use 1290 Infinity III Systems and dedicated 2D-LC valve technology for UHPLC performance, fast gradients, high sensitivity, and excellent robustness
  • Combine first and second dimension solvents and gradients as you like using Agilent Active Solvent Modulation Technology
  • Replace multiple one-dimensional separations by one two-dimensional separation for better results in less time
  • Upgrade your Agilent 1290 Infinity III LC System (1-D) easily to 2D-LC system for better resolution
  • Use the 1290 Infinity III Bio 2D-LC system for applications in biopharma, pharma, chemical and other industries that use samples, that require minimum surface binding and metal ion elution or use solvents or reagents over a wide pH range or with high salt concentrations. Materials like MP35N, ceramics, PEEK or gold are known for excellent characteristics under such conditions and are used in the entire flow path of Agilent biocompatible LC systems.
  • Learn basic and advanced 2D-LC functionality with the Agilent Easy Start Service to realize the full power of 2D-LC. Key qualitative and quantitative workflows are explained and demonstrated in the customer’s lab. This includes 2D method setup and development, data analysis and reporting, system configuration and instrument control. Easy Start Service is available in 2-or 3-day flavors covering one or multiple workflows for different software products including 2D-LC Software for OpenLab CDS, MassHunter and ChemStation and GC Image Software LC x LC Edition.
  • Start working with 2D-LC today using application notes in many areas such as pharmaceuticals, biopharmaceuticals, chemicals, fuels, food, and many others
  • Your choice for performance and sustainability: The 1290 Infinity III LC System has received the My Green Lab ACT (Accountability, Consistency, Transparency) label after an independent audit to verify its environmental impact throughout the product life cycle.

Brochures

Product brochuresSeparations

Simplify complex separations, fast

In this product brochure, take a closer look at Agilent’s InfinityLab 2D-LC Solutions for complex separations. These solutions from Agilent are designed to be universally applicable to a wide variety of challenging separations and promise to deliver the results you need fast and efficiently.



Application NoteSeparations

Protein A Affinity IgG Capture Followed by AdvanceBio SEC Aggregation Analysis

This application note describes the quantification of aggregates in biotherapeutic samples using the Agilent InfinityLab 2D-LC solutions. Protein A affinity capture was performed in the first dimension, and size exclusion chromatography in the second dimension. Multiple heart-cutting was used to transfer the samples from the first to the second dimension. This technique enabled the entire characterization to be done with a single analysis, eliminating the need for fraction collection and multiple injections.

Agilent Technologies' 2D-LC and IMS-QTof Instrumentation for High-Throughput, Non-Targeted Screening of Complex Samples

Find out how the separation power of Agilent’s 2D-LC solution, together with the ion mobility-capability of the Agilent 6560 Ion Mobility Q-TOF LC/MS, are ideal for the analysis of isobaric compounds, like those commonly found in Chinese medicine, and how the speed and ease of operation means several thousands of compounds can be analyzed in a single run, in one hour.


Fast Method Optimization and Simple Data Analysis using 2D-LC

In this video, learn how Dr Matthias Pursch, of The Dow Chemical Company, uses Agilent's multi-heart-cutting 2D-LC and comprehensive 2D-LC solution for better resolution and quantitation, and the intuitive operation software for faster method development and higher throughput.










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